超临界色谱拆分6种唑类手性农药

Enantiomer resolution of six triazole with supercritical fluid chromatography

采用超临界流体色谱(SFC),在Chiralpak IB(一种新型的纤维素衍生物键合型手性固定相)上对三唑酮、己唑醇、戊唑醇、烯效唑、腈菌唑以及恶醚唑等6种三唑类杀菌剂进行了手性拆分,并分析讨论了甲醇、乙醇和异丙醇三种醇类流动相改性剂对手性农药分离的影响.实验结果表明,除恶醚唑外,其他5种农药手性对映体都获得了基线分离,且大部分的手性分离时间在10 min以内.改性剂的浓度和种类对手性分离的保留时间和分离都有一定影响,不同的改性剂适于拆分不同的农药.

Enantiomeric separation of six triazole fungicides： triadimefon,hexaconazole,tebuconazole,difenoconazole,uniconazole,myclobutanil,was conducted on chiral column Chiralpak IB（a new chiral stationary phase containing cellulose tris（3,5-dimethylphenylcara bamate） immobilized onto silica gel） using supercritical fluid chromatography,and the influence of alcohol modifiers methanol,ethanol and isopropanol in mobile phase（super-critical CO2） on the enantio-separation was studied.All fungicides,except difenoconazole,were obtained baseline separation;and in most cases the retention time was shorter than 10min.The retention and resolution of the samples were obviously influenced by the concentration and type of the modifier.The separation results were satisfactory because the modifier showed good selectivity for the chiral pesticide to be separated.