板蓝根抗甲1型流感病毒活性物质的初筛研究

Screening of Anti-H1N1 Active Constituents from Radix Isatidis

【目的】观察不同提取工艺及方法制备的板蓝根样品的抗流感病毒作用,以筛选板蓝根中抗流感病毒的有效成分及活性物质。【方法】于狗肾细胞(MDCK)上接种人甲1型流感病毒PR8株(A/PR/8/34,H1N1),采用细胞病变抑制法(CPE法),在不同试验策略下(保护、治疗模式)评价不同提取工艺及方法制备的板蓝根样品的体外抗流感病毒作用。【结果】在无毒浓度下,从不同工艺制备的板蓝根样品中筛选到1份有抗流感病毒活性的成分,该成分为醇溶液通过大孔树脂用纯水洗脱的洗脱液(S-03),其体外抗病毒选择指数为4.8。【结论】发现板蓝根样品中抗流感病毒作用较为明确的成分。

Objective The anti-influenza activity of Radix Isatidis extracts processed by different extractions was observed for the screening of anti-influenza active constituents.Methods Madin-Darby canine kidney cell line was inoculated with influenza virus type 1 PR8 strain（A/PR/8/34,H1N1）.The anti-influenza activity of Radix Isatidis extracts processed by different extractions was evaluated according to cytopathic effect（CPE）.Results At non-toxic concentration,one antiviral active constituent（S-03） was obtained from different extractives of Radix Isatidis.S-03 was the eluant eluted with pure water through the macroporous resin column,and its anti-viral selective index（SI） was 4.8 in vitro.Conclusion One antiviral constituent has been found in Radix Isatidis extracts.