体外培养人黑素细胞HLA-DR抗原的表达

THE HLA-DR antigen expression on human melanocytes in vitro and after growth in culture

为了解正常人表皮黑素细胞是否表达HLA DR抗原 ,黑素细胞经体外培养后 ,是否因细胞培养的添加成分或培养时间的长短而影响LHA DR抗原表达而应用免疫酶染色法进行检测。结果发现表皮基底层的黑素细胞不表达HLA DR抗原 ;接种于含TPA培养液的黑素细胞 ,在传代后2周 ,黑素细胞表达HLA DR抗原 ,7周后HLA DR抗原失表达 ;接种于不含TPA培养液的黑素细胞 ,在传代后2周和7周时均不表达HLA DR抗原。因此 ,体外培养的黑素细胞表达HLA DR抗原是因为培养液中含有促分裂剂或某种细胞因子 ,而正常人的黑素细胞是不表达HLA DR抗原的。这就为体外培养纯黑素细胞的同种异体移植提供了可能。

To study whether normal human melanocytes express HLA DR antigen and whether HLA DR expression of melanocytes altered after prolonged in vitro culture, ABC methods were used in the study. The results indicated that the melanocytes in vivo did not express HLA DR antigen, and in vitro the expression of HLA DR antigen was due to the presence of mitogen or a certain kind of cytokine in the culture medium. Thus it may provide a possibility that the allograft of cultured melanocytes could be used in the treatment of vitiligo.