摘要
目的探讨用白介素-8（IL－8）作配体，检测红细胞趋化因子受体（ECKR）结合活性及其临床意义。方法分离的红细胞与一定量的IL-8溶液结合，离心后取上清液用酶联免疫吸附试验（ELISA）检测IL－8含量，反映ECKR结合活性。结果34例正常人ECKR(红细胞数为3．0×1012／L）结合活性（±s％）为37．6±7．8，30例肝炎、31例肺癌、16例慢性肾功能衰竭和8例系统性红斑狼疮患者则分别为19．7±10．5、19．8±10．9、21．5±6．4和21．3±12．2。经统计，各疾病组ECKR结合活性明显低于正常人组（P＜0．01）。结论红细胞参与趋化因子的调控，在上述疾病的发病过程中可能起重要作用。临床上检测ECKR结合活性有其临床应用价值。
： Objective To study the detection and its clinical significance of the erythrocyte chemokine recepter(ECKR)binding activity with interleukin - 8(IL - 8 ) as ligand. Methed Isolated erythrocytes bound to IL - 8. After centrifuge IL - 8 levels were measured with enzyme linked immunosorbent assay(ELISA)in the supernatant. Results In 34 mormal adults, the ECKR(erythrocytes count 3. 0× 1012/L)binding activity (±s% )was 37. 6±7. 8, in 30 hepatitis, 31 lungcancer, 16 CRF and 8 SLE, it was 19. 7±10. 5、19. 8±10. 9、21. 5±6. 4and 21. 3± l2. 2 respectively. The ECKR binding activity of the disease groups deced signifcantly (P<0. 01 )compared with normal contro1 guoup by statistics. Chnclusion The erythrocytes paticipate in the chernchine regulation and may play a novel role in the pathogenesis of aha diseases. The detectbo of the ECKR binding activity has a value of clinic application.[
出处
《深圳中西医结合杂志》
1999年第5期10-13,共4页
Shenzhen Journal of Integrated Traditional Chinese and Western Medicine
关键词
红细胞趋化因子
受体
ECKR
IL-8
ELISA
Erythrocyte chemokine receptor
Interleukin-8
Enzyme linked immunosorbent assay
Erythrocyte immune
