摘要
目的研究不同浓度葡萄糖和胰岛素对3T3-L1脂肪细胞中内脏脂肪素(Visfatin)mRNA表达的影响。方法通过real-time RT-PCR方法检测不同浓度葡萄糖和胰岛素培养下3T3-L1脂肪细胞Visfatin mRNA的表达。结果葡萄糖增加了3T3-L1脂肪细胞Visfatin mRNA的表达;胰岛素降低其表达。结论葡萄糖和胰岛素对3T3-L1脂肪细胞中Visfatin mRNA的表达有调控作用。
Objective To investigate the effects of glucose and insulin in different concentrations on the expressions of visfatin mRNA in 3T3-L1 adipocytes. Methods 3T3-L1 adipocytes were treated with different concentration of glucose and insulin. The expressions of visfatin mRNA were measured by quantitative real-time RT-PCR with 36B4 as internal control. Results Glucose increased the expression of visfatin mRNA, hut insulin downregulated visfatin mRNA expressions in 3T3-L1 adipocytes. Conclusions Glucose and insulin regulate the expression of visfatin mRNA in 3T3-L1 adipocytes.
出处
《中国糖尿病杂志》
CAS
CSCD
北大核心
2011年第7期542-544,共3页
Chinese Journal of Diabetes
基金
教育部高等学校博士学科点科研基金(200802260007)
黑龙江省教育厅高等学校骨干教师创新计划(1054C3024)
