解脲脲原体小鼠宫内感染的实验研究

EXPERIMENTAL STUDY ON THE INTRAUTERINE INFECTION WITH UREAPLASMA UREALYTICUM IN MICE

目的 建立解脲脲原体（Ｕｕ）小鼠宫内感染的动物模型。方法 分别将４、１ 和９ 型Ｕｕ（２×１０４ ｃｆｕ／ｍ ｌ）接种于妊娠第１２．５、１３．５、１４．５ 天孕鼠阴道内（３４ 只），以最后一次攻击后的４８ｈ 内分娩为早产。以腹腔内接种４型Ｕｕ（６ 只）和阴道内注入灭菌盐水（９ 只）为对照组。结果 经阴道内接种Ｕｕ４型的４３．７％ （７／１６）孕鼠在４８ｈ 内早产，显著高于１ 型Ｕｕ（８．３％ ，１／１２，Ｐ＜ ０．０５）和９型Ｕｕ（０％ ，０／６，Ｐ＜ ０．０５）以及对照组（０％ ，０／１５，Ｐ＜ ０．０１）；接种４、１ 和９型Ｕｕ 后，早产和不早产孕鼠子宫角Ｕｕ 的检出率分别为９７％ 、４６％ 和２５％ ；接种４ 型Ｕｕ 后，早产幼鼠的心、肺Ｕｕ 检出率为４６％ （２５／５４），而注射灭菌盐水、１和９ 型Ｕｕ 的非早产幼鼠中Ｕｕ 的检出率均为０％ （０／４５，０／５５，０／４５，Ｐ＜ ０．０１）。三种血清型Ｕｕ 感染后早产或不早产幼鼠脑组织中Ｕｕ 的检测均为０％ （０／５９）。结论 小鼠Ｕｕ４ 型和１型阴道内接种可导致部分孕鼠宫内感染动物模型的建立将有助于对人类Ｕｕ 早产发病机制的进一步研究。

Aim\ To establish model of intrauterial infection with U.urealyticum in mice.Methods\ Forty nine famale mice at 75% of the length of the gestational period(12 5、13 5、14 5 days,respectiviy)received either an intravaginal inoculum of 2×10 4 cfu/ml U.urealyticum(n=6),or an intravaginal injection of a sterile physiological salt solution(n=9).Results\ Delivery within 48 hours of the procedure occurred in 43 7% of mice after intravaginal inoculum serotype 4,in 0% after intraperitioneal U.urealyticum(P<0.05),and in 0% after intravaginal injection of sterile saline(P<0.01).Intravaginal injection of serotype 1 and 9 resulted in delivery rates of 8 3%(1/12,P<0.05) and 0%(0/6,P<0.05) respectively;U.urealyticum inoculation produced uterine horn infection rates of 97%、46% and 25% by serotype 4、1 and 9 respectively.Serotype 4 intravaginal inoculation produced systemic infection of heart and lung in 46%(25/54) of baby mice,while in 0%(0/45,0/55,0/45) after sterile saline intravaginal injection,and serotype 1,serotype 9,respectively(P<0 001).The detection rate of U.urealyticum was 0%(0/59) in brain tissue of both premature delivery and full term mice.Conclusions\ A model of intravaginal inoculation with U.urealyticum in mice leads to intrauterine infection,and may be helpful for researchers investigating the pathophysiologic features in human preterm labor.