肺部炎性疾病者生殖支原体套式PCR检测研究

DETECTION OF MYCOPLASMA GENITALIUM BY NESTED POLYMERASE CHAIN REACTION IN PULMONARY INFLAMMATION DISEASE IN PEDIATRIC PATIENT

目的 探讨生殖支原体（Ｍｇ）与肺部炎性疾病的关系。方法 应用原核生物通用引物ＰＣＲ直接测序克隆Ｍｇ１６ＳｒＲＮＡ基因片段序列，自行建立Ｍｇ 套式ＰＣＲ扩增法，检测健康儿童和肺部炎性疾病患儿咽拭子标本中的Ｍｇ－ ＤＮＡ。结果 健康儿童Ｍｇ 阳性率为５％ ，儿童肺炎、儿童支气管炎、新生儿肺炎者阳性率分别为２１．６％ 、２８．６％ 、２０％ （Ｐ均＜ ０．０１）。结论 Ｍｇ

Aim\ To explore the relationship between mycoplasma genitalium(Mg) and pulmonary inflammation disease.Method\ The fregment of Mg-16SrRNA gene was cloned and an nested polymerase chain reaction(Nested-PCR)was established.Result\ The positive rate of Mg-DNA Nested-PCR from throat swab was 5%,21 6%,28 6%,20% in health,pneumonic,bronchitic children and pneumonic newborn infants.Mg-DNA detective rate was significantly higher in case group than the control group(all P<0 01).Conclusion\ There may be a strong association between Mg and pulmonary inflammaton disease.