Octreotide治疗Graves’眼病机理探讨

Investigation of the mechanism by which octreotide acts on Graves' Ophthalmopathy

目的 探讨Ｏｃｔｒｅｏｔｉｄｅ 对细胞因子刺激后的人眼球后成纤维细胞的细胞间粘附分子１（ＩＣＡＭ１） 表达及ＤＮＡ 合成的影响。方法 应用细胞培养技术， 测定加入细胞因子及Ｏｃｔｒｅｏｔｉｄｅ后ＩＣＡＭ１ 表达和ＤＮＡ 合成情况。结果 ＴＮＦα（１０３ Ｕ／ｍｌ） 、γＩＮＦ（１００ Ｕ／ｍｌ） 和ＩＬ１β（１００ Ｕ／ｍｌ） 均能强烈刺激眼球后成纤维细胞表面ＩＣＡＭ１ 表达，而低浓度（ 即药理浓度）Ｏｃｔｒｅｏｔｉｄｅ 能明显地抑制这种刺激作用，但所需浓度及发挥抑制作用的时间各不相同；同样，低浓度Ｏｃｔｒｅｏｔｉｄｅ 也能抑制细胞因子（除ＩＬ１β外） 刺激的ＤＮＡ 合成。结论 Ｏｃｔｒｅｏｔｉｄｅ 治疗Ｇｒａｖｅｓ’眼病的作用途径部分是通过抑制细胞因子的作用，使ＩＣＡＭ１ 表达及ＤＮＡ 合成减少，它可能具有免疫调节的特性。

Objective Effects of octreotide on intercellular adhesion molecule 1 (ICAM 1) expression and DNA synthesis in cultured retroocular fibroblasts following stimulation by cytokines. Methods With cytokines and octreotide added to cultured retroocular fibroblasts, ICAM 1 expression and DNA synthesis were measured. Results Some cytokines, such as TNF α (10 3U/ml), γ INF (100U/ml) and IL 1β (100U/ml) significantly stimulated the ICAM 1 expression by retroocular fibroblasts. Octreotide at low concentration markedly inhibited this stimulating effect. But the concentration and time needed for the inhibition were different. Octreotide in low concentration also inhibited the DNA synthesis which had been stimulated by some cytokines (except IL 1β). Conclusion The mechanism by which octreotide acts on Graves' ophthalmopathy is in part by inhibiting the effect of cytokines. Octreotide may have immunomodulatory properties.