Endostatin抑制高转移性人肝癌皮下移植瘤的生长

Inhibition of growth of LCI D20 hepatocellular carcinoma by Endostatin

目的 探讨Ｅｎｄｏｓｔａｔｉｎ 对高转移潜能肝癌ＬＣＩＤ２０ 生长的抑制作用。方法 ＬＣＩＤ２０ 组织植入裸鼠皮下，分别采用不同剂量的Ｅｎｄｏｓｔａｔｉｎ、顺铂以及二者联合治疗。每５ 天测量肿瘤的长短径。结果 ２ ｍｇ·ｋｇ－ １·ｄ－ １ 的Ｅｎｄｏｓｔａｔｉｎ 对肿瘤生长无作用，４ ｍｇ·ｋｇ－１·ｄ－ １ 的Ｅｎｄｏｓｔａｔｉｎ 和１ ｍｇ·ｋｇ－ １·ｄ－ １的顺铂对肿瘤生长有抑制作用，而联合用药组对肿瘤生长的抑制更强。结论 足量血管形成抑制剂可抑制肝癌生长；与细胞毒性药物联合应用可提高疗效。

Objective\ To evaluate the inhibitory effect of endostatin on the growth of LCI D20, a highly metastatic hepatocellular carcinoma. Methods\ Thirty nude mice were subcutaneously implanted with LCI D20 tumor tissues, then treated with subcutaneous injection of 2 mg·kg -1 ·d -1 (group EA) or 4 mg·kg -1 ·d -1 (group EB) Endostatin, or intraperitoneal injection of 1 mg·kg -1 ·d -1 cis-platin (group C), or 4 mg·kg -1 ·d -1 plus 1 mg·kg -1 ·d -1 cis-platin (group EC), or subcutaneous injection of 0.2 mg/d PBS as control. All treatment lasted 40 days after implantation. The tumor size was calculated by using the formula: V=L×W 2×0.52. Results\ The mean tumor size of each group was (8376±2129) mm 3 for the control, (6095±2261) mm 3 for group EA, (3777±2042) mm 3 for group EB, (1629±877) mm 3 for group C, and (1248±847) mm 3 for group EC, when measured on the day 40 after implantation. Statistical analysis showed that 2 mg·kg -1 ·d -1 Endostatin had no inhibitory effect on the growth of LCI D20, however, 4 mg·kg -1 ·d -1 Endostatin, 1 mg·kg -1 ·d -1 cis-platin and combination of these two drugs delayed the growth of LCI D20. Combination treatment increased the inhibitory effect on the tumor growth, as compared with Endostatin alone. Conclusion\ Antiangiogenic therapy inhibited the growth of hepatocellular carcinoma. Combination treatment improved the inhibitory effect.\;