类胰岛素生长因子1对转化生长因子β_1诱导前列腺增生间质细胞类胰岛素生长因子结合蛋白3生成的抑制作用

The inhibitory effects of insulin like growth factor 1 on the induction of IGFBP 3 in prostate stromal cell by TGFβ_1

目的 探讨类胰岛素生长因子（ＩＧＦ１） 和转化生长因子（ＴＧＦβ１） 在调节前列腺间质细胞ＩＧＦ结合蛋白———类胰岛素生长因子结合蛋白３（ＩＧＦＢＰ３） 中的作用。方法 前列腺增生组织原代培养分离间质细胞。分别用ＴＧＦβ１（５ μｇ／Ｌ）、ＩＧＦ１（５００ μｇ／Ｌ） 及ＴＧＦβ１＋ ＩＧＦ１ 处理间质细胞。Ｗｅｓｔｅｒｎ ｂｌｏｔ检测间质细胞内的ＩＧＦＢＰ３ 水平。结果 间质细胞用ＴＧＦβ１ 处理２ 小时后，ＩＧＦＢＰ３ 比对照组增加１３２ ％ ，４ 小时后比对照组高出２５１％ ，８ 小时后接近原来水平。ＩＧＦ１ 可以明显逆转ＴＧＦβ１ 诱导增加的ＩＧＦＢＰ３，而单纯ＩＧＦ１ 则对间质细胞的ＩＧＦＢＰ３ 水平没有明显的影响。结论 ＩＧＦ１ 有抑制ＴＧＦβ１ 诱导前列腺间质细胞ＩＧＦＢＰ３ 表达的作用。

Objective\ To investigate the role of insulin like growth factor 1 (IGF1) and TGFβ1 in the regulation of IGFBP 3 in prostate stromal cells. Methods\ Primary culture stromal cells derived from benign prostate hyperplasia patients were treated with TGFβ1 (5 ng/ml), IGF1 (500 ng/ml) and TGFβ1 plus IGF1 respectively. Western blot was used to detect the expression of IGRBP 3 in the stromal cells. Results\ Compared to the control cells IGFBP 3 was increased by 132 % at 2 h and 251 % at 4 h following treatment with TGFβ1. Increasing IGRBP 3 level induced by TGFβ1 was reversed by IGF1 but IGF1 alone had no significant effect on the IGFBP 3 level in prostate stromal cells. Conclusion\ IGF1 can significantly inhibit the induction of IGFBP 3 in prostate stroma cells by TGFβ1.\;