动脉硬化闭塞症与血管内皮细胞活性因子关系的研究

Study on the relationship between endothelial activity factors and atherosclerosis obliterans

目的 探讨动脉硬化闭塞症（ＡＳＯ） 与内皮素１（ＥＴ１） 、一氧化氮（ＮＯ） 等血管内皮细胞活性因子的相互关系。方法 选择９６ 例ＡＳＯ 患者，采用密度梯度法检测循环内皮细胞计数（ＣＥＣ），采用放射免疫测定法检测血浆ＥＴ１ 、血栓素Ｂ２（ＴＸＢ２）、６酮前列腺素Ｆ１α（６ＫＰＧＦ１α） ，降钙素基因相关肽（ＣＧＲＰ） 、血管紧张素Ⅱ（ＡＴⅡ）、肿瘤坏死因子（ＴＮＦ），采用酶联免疫检测细胞间粘附分子１（ＩＣＡＭ１）、Ｐ选择素（ＰＳ）。采用Ｇｒｉｓｓ 法检测血浆ＮＯ水平。结果 所有患者均存在高ＥＴ１、ＴＸＢ２ 、ＡＴⅡ、粘附分子、ＣＥＣ血症，存在低ＮＯ、ＣＧＲＰ、６ＫＰＧＦ１α血症，且以Ⅲ期病人最明显。结论 ＡＳＯ 的发生发展同血管内皮细胞活性因子密切相关，ＥＴ１、ＮＯ 等血管活性因子的检测有助于ＡＳＯ发病机制的探讨和病情严重程度的判别。

Objective To investigate the relationship between endothelial activity factors and atherosclerosis obliterans(ASO). Methods The plasma levels of endothelins 1 (ET 1), thromboxane B 2 (TXB 2), 6 ketto prostaglandin F1α (6 K PGF1α), calcium genetic related peptide (CGRP), tumor necrosis factor (TNF), intercellular adhesion molecule 1 (ICAM 1), P selectin (P S), angiotension Ⅱ (AT Ⅱ) were determined by using radioimmunoassy, Griss method and enzyme immunoassay respectively in 98 patients with ASO. Results The ASO patients had high levels of plasma ET 1, TXB2 and AT Ⅱ, low levels of plasma NO, CGRP and 6 K PGF1α, which were closely related with the stages of ASO. Conclusion The dysfunction of vascular endothelial cells was closely related with development of ASO. The determination of such vascular endothelial activity factor as ET 1, NO, etc. is helpful to investigate the mechanism of ASO and judge the severity of ASO.