碳酸酐酶Ⅰ、Ⅱ在结直肠癌中的表达及意义

Expressions and Significances of Carbonic AnhydraseⅠand Ⅱin Colorectal Cancer

目的探讨碳酸酐酶Ⅰ、Ⅱ(CAⅠ、CAⅡ)的表达与结直肠癌发生、发展以及临床病理特征的关系。方法以免疫组织化学法检测CAⅠ、CAⅡ在配对的55个正常结直肠黏膜、27个息肉、64个结直肠癌及20个转移淋巴结中的表达,比较各组间表达差异。分析CAⅠ、CAⅡ的表达与结直肠癌的分化、临床病理分期和淋巴结转移(LNM)之间的关系。结果 CAⅠ、CAⅡ的表达在息肉、结直肠癌中逐步降低,与正常黏膜比较,差异有统计学意义(P<0.05),在转移淋巴结中表达与配对原发灶相似(P>0.05)。CAⅠ、CAⅡ在结直肠癌中的表达与分化程度和TNM分期无关(P>0.05)。结论 CAⅠ、CAⅡ的表达下调是结直肠癌发生中的早期事件,与淋巴结转移无关。

Objective To investigate the expressions of carbonic anhydrases Ⅰ and Ⅱ（CAⅠ,CAⅡ） in colorectal cancer（CRC）,and their relationship with oncogenesis,progression and clinical pathological characters of CRC.Methods The expressions of CAⅠand CAⅡ were detected in paired samples of 55 normal colorectal mucosas,27 polyps,64 CRCs and 20 lymph node metastases（LNM）.Differences among them were compared.The relationships of CAⅠ and CAⅡ expressions with differentiation,TNM stages and LNM were also analyzed.Results Expressions of CAⅠand CAⅡ were significantly down-regulated stepwise from normal colorectal mucosa,polyp to cancer（P 0.05）.Expressions of CAⅠ and CAⅡ in lymph node metastasis were similar to the paired primary cancer （P 0.05）.Expressions of CAⅠand CAⅡ in CRC had no correlation with cancer differentiation or TNM stages （P 0.05）.Conclusion The down-regulations of CAⅠand CAⅡ are early incidents during oncogenesis of CRC and have no correlation with LNM.