HSS介导依托泊甙对人肝细胞癌裸鼠移植瘤的抑制作用

Inhibitory effect of HSS mediated etoposide on xenograft hepatocellular carcinoma in nude mice

目的：了解肝细胞生长刺激物质（ＨＳＳ）介导依托泊甙（ＶＰ－１６）对肝癌裸鼠移植瘤的抑制作用。方法：建立人肝细胞癌裸鼠移植瘤模型，对荷瘤小鼠腹腔内注射不同剂量ＶＰ－１６及ＨＳＳ治疗２周；治疗结束后，于不同时间处死动物，进行病理学检查，并计算肿瘤生长抑制率。结果：与对照组相比，中、大剂量ＶＰ－１６（０．７５，１．５ μｇ／ｇ）联合ＨＳＳ（１ μｇ／ｇ）组肿瘤生长明显受抑，抑瘤率分别为７１％ 和６９％ （Ｐ＜ ０．０１），但中、小剂量组之间差异无显著性（Ｐ＞ ０．０５）；而小剂量联合（ＶＰ－１６ ０．３８ μｇ／ｇ）组及单用ＨＳＳ（１ μｇ／ｇ）组较对照组无明显差异（Ｐ＞ ０．０５），抑瘤率分别为２３％ 和５％ 。单用大剂量ＶＰ－１６副作用明显。中、大剂量ＶＰ－１６联合ＨＳＳ均可显著延长小鼠存活期。结论：ＨＳＳ介导大、中剂量ＶＰ－１６具有明显抑瘤效应，以中剂量为佳。

Objective: To observe the inhibitory effect of hepatocyte stimulating substance(HSS) mediated etoposide(VP 16)on xenograft hepatocellular carcinoma in nude mice. Methods: VP 16 + HSS was injected into the peritoneal cavity of nude mice with xenograft hepatocellular carcinoma. The pathological changes were observed, and tumor growth inhibitory rate was calculated. Results: The tumor growth inhibitory rate in middle and large dosage of VP 16(0.75, 1.5 μg/g)+HSS(1 μg/g) group were 71% and 69%,respectively( P <0.01). Small dosage combined group(VP 16 0.38 μg/g) and single HSS group were 23% and 5%( P >0.05), respectively. Large dosage of VP 16(1.5 μg/g) resulted in side effect severely. The combination of VP 16 with HSS in middle dose had longer survival time. Conclusion: The effect of VP 16 in combination with HSS might improve the tumor inhibitory effect significantly.