子宫内膜异位症患者腹腔液体外对NK细胞活性的影响

Effect of peritoneal fluid of women with endometriosis on the natural killer cell activity in vitro

目的：探讨子宫内膜异位症（ＥＭ）患者的腹腔液（ＰＦ）对自然杀伤（ＮＫ）细胞的作用。方法：以Ｈｅｌａ细胞作为靶细胞，采用ＬＤＨ释放法测定与ＥＭ 患者ＰＦ共同孵育后正常人血ＮＫ细胞活性的变化。结果：在作用２ ｈ 时，非ＥＭ 患者和ＥＭ 患者ＰＦ对正常人外周血ＮＫ 细胞活性的抑制率分别为（３７．１±１８．９）％ 和（６５．２±２１．８）％ ，两者相差非常显著（Ｐ＜ ０．０１）。抑制率与ＥＭ 临床分期呈显著正相关（ｒ＝ ０．６０２ ３， Ｐ＜ ０．０５）。延长ＰＦ与淋巴细胞的作用时间，则ＰＦ对ＮＫ 细胞活性的抑制率也随之升高。结论：ＥＭ 患者的ＰＦ对ＮＫ 细胞活性具有明显的抑制作用。

Objective: To investigate the effect of the peritoneal fluid (PF) of women with endometriosis(EM) on the natural killer (NK) cell activity. Methods: Hela cells being target cell, the LDH releasing assay was applied for measurement of the change of NK cell activity after preincubation with PF of EM. Results: After incubated with PF of women with and without EM for two hours, the inhibition percentages of NK cell activity was decreased by (37.1±18.9)% and (65.2±21.8)% ,respectively. Their difference was significant statistically ( P <0.01). The inhibition percentage was correlated with the severity of EM ( r=0.602 3, P <0.05). Prolonged the incubation time, the NK cell activity was decreased more significantly. Conclusion: PF of EM can significantly decrease NK cell activity in vitro .