腮腺淋巴上皮瘤样癌与EB病毒感染的关系(英文)

Association of Epstein Barr virus with lymphoepithelioma like carcinoma of the parotid gland 

目的：研究ＥｐｓｔｅｉｎＢａｒｒ 病毒（ＥＢＶ）与腮腺淋巴上皮瘤样癌（ｌｙｍｐｈｏｅｐｉｔｈｅｌｉｏｍａｌｉｋｅｃａｒｃｉｎｏｍａ，ＬＥＬＣ）的关系，并检测瘤细胞内ＥＢＶ 基因编码产物。方法：作者收集了中山医科大学所属病理科１９８６ 年１ 月至１９９５ 年１２ 月间３２ 例腮腺ＬＥＬＣｓ．。３２ 例ＬＥＬＣ石蜡包埋标本再次切片。采用免疫组化和原位核酸杂交法检测瘤细胞内ＥＢＶ 基因表达产物。结果：（１） 在１２５例腮腺癌中有３２ 例淋巴上皮瘤样癌，占总病例的２５－６％ （３２／１２５） 。（２）所有３２ 例腮腺ＬＥＬＣ组织中均有数量不等的ＥＢＮＡ１ 和ＥＢＥＲｓ 阳性瘤细胞。（３）２７ 例ＬＥＬＣ 组织中部分瘤细胞表达ＬＭＰ１ 。（４） 所有标本中均未见ＺＥＢＲＡ 阳性细胞。（５）３２例腮腺ＬＥＬＣ组织中ＥＡＤ、ＶＣＡ和ＭＡ的阳性表达率分别为７１－９ ％（２３／３２） 、６８－８ ％（２２／３２） 和１２－５％ （４／３２） 。结论：（１） 在鼻咽癌高发的广州地区，腮腺ＬＥＬＣ的发病率也较高。（２）腮腺ＬＥＬＣ 组织中均有ＥＢ病毒感染。（３）ＥＢ病毒在腮腺ＬＥＬＣ的感染主要为潜伏Ⅱ型，即表达ＥＢＮＡ１ 、ＥＢＥＲｓ

Purpose To investigate the relationship between Epstein Barr virus (EBV) and lymphoepithelioma like carcinoma (LELC) of the parotid gland and detect the gene expression products of EBV harbouring in LELC cells. Methods Thirty two parotid LELCs were collected from the Departments of Pathology, Sun Yat sen University of Medical Sciences, Guangzhou, China during the period of January 1986 and December 1995. All the 32 formalin fixed paraffin embedded blocks had been consecutively re sectioned again. Immunohistochemical and in situ nucleic acid hybridization methods for detection of EBV gene encoded products were performed. Results (1) 32 LELCs were found out of 125 parotid gland carcinomas, the frequency was 25.6% (32/125). (2) All of the 32 specimens contained a variable number of EBNA 1 and EBERs positive neoplastic cells. (3) Twenty seven out of 32 specimens (27/32, 84 4%) had a portion of carcinoma cells expressing LMP 1. (4) No ZEBRA positive cell could be found. (5) EA D, VCA and MA positivity rates for these 32 parotid LELCs reached to 71 9%(23/32), 68 8%(22/32), and 12 5%(4/32), respectively. Conclusions (1) The parotid gland LELC is frequently to be seen in Guangzhou locale of China, where is a high incidence area of nasopharyngeal carcinoma (NPC). The parotid gland LELC and NPC are co prevalent in Guangzhou locale. (2) This disease is also consistently associated with EBV infection. (3) The EBV infection of the parotid gland LELCs is essentially the type of latency Ⅱ, expressing EBNA 1, EBERs and LMP 1. (4) The latent infected EBV harbouring in LELC cells could in part be switched over to lytic cycle, producing EA D, VCA or/and MA.