吉兰-巴雷综合征外周血单个核细胞DcR3 mRNA表达

The expression of DcR3 mRNA in peripheral blood mononuclear cells from patients with Guillain-Barré syndrome

目的研究吉兰-巴雷综合征(Guillain-Barrésyndrome,GBS)患者外周血单个核细胞(PBMCs)中凋亡相关基因诱骗受体(DCR3)mRNA的表达水平。探讨DcR3与GBS疾病严重程度及脑脊液免疫球蛋白(CSF-Ig,包括IgGI、gA、IgM)水平之间的关联性。方法以36例GBS患者和20名健康人为研究对象,应用反转录-聚合酶链反应(RT-PCR)检测外周静脉血PBMCs DcR3 mRNA表达水平,并对GBS患者CSF-Ig水平与疾病严重程度和DcR3 mRNA表达的相关性进行分析。结果 GBS患者PBMCs中DcR3 mRNA表达水平(0.40±0.08)明显高于健康对照组(0.17±0.04)(P<0.01),但轻型组和重型组患者表达水平(分别为0.39±0.07、0.41±0.05)间比较无统计学差异(P>0.05)。GBS患者轻型组IgG、IgA、IgM表达水平分别为(0.123±0.113)、(0.016±0.012)和(0.012±0.011)g/L,与重型组〔IgGI、gA、IgM分别为(0.128±0.112)(、0.015±0.011)和(0.013±0.013)g/L〕比较无统计学差异(均P>0.05)。DcR3 mRNA表达与CSF-Ig水平异常无相关性(rIgG=0.0083,rIgA=0.0056,rIgM=0.0015,均P>0.05)。结论 GBS患者PBMCs DcR3 mRNA表达增加,提示DcR3可能与GBS免疫细胞凋亡异常和免疫调节紊乱有关,可能参与了GBS的发病过程。DcR3与GBS病情严重程度和CSF-Ig的异常无相关性。

Objective To investigate the expression of DcR3 mRNA in peripheral blood mononuclear cells （PBMCs） from patients with Guillain-Barre syndrome （GBS）. And to discuss the correlation among cerebrospinal fluid （CSF） -Ig, the severity of GBS and DcR3 mRNA. Methods Thirty-six patients with GBS and 20 healthy controls were included. The expression of DcR3 mRNA in PBMCs was detected by reverse transcription-polymerase chain reaction （RT-PCR）. The correlation between the severity of GBS and the expression of DcR3 mRNA was analyzed. The CSF level of CSF-Ig was also analyzed. Results DcR3 mRNA level in GBS patients （0.40±0.08） was significantly higher than in the healthy control （0.17 ± 0.04） （P〈 0.01）. The expression of DcR3 mRNA was comparable between the mild group （0.39±0.07） and the severe group （0.41±0.05） （P〉0.05）. The level of CSF-Ig was also comparable between the mild group [IgG （0. 123 ±0.113） g/L, IgA （0.016±0.012） g/L, IgM （0.012±0.011） g/L] and the severe group [IgG （0.128±0.112） g/L, IgA （0.015±0.011） g/L, IgM （0.013±0.013） g/L] （P〉0. 05）. There was no correlation between the levels of CSF-Ig and DcR3 mRNA （rlgG= 0. 0083, rlgA = 0. 0056, rlgM=0. 0015, P〉0.05）. Conclusions The expression of DcR3 mRNA in PBMCs increased significantly in GBS patients, indicating that DcR3 could be involved in the pathogenesis of GBS. There was no correlation between GBS severity and DcR3 mRNA level or between CSF-Ig and DcR3 mRNA levels.