离子交换树脂分离发酵新工艺生产L-苹果酸的研究

Study on the Production of L-Malic Acid by a New Technology of Extractive Fermentation Using Ion Exchange Resin

研究开发一种L-苹果酸发酵的新工艺——离子交换树脂分离发酵法。确定了最佳工艺条件,其中,分离发酵起点为36h,稀释率为0.15h-1,分离发酵周期为96h。与常规分批发酵相比,采用分离发酵法生产L-苹果酸,能明显提高菌体的糖转化率和产酸速率,并显著缩短发酵周期。

A new technology, extractive fermentation using ion exchange resin, for fermentation of L malic acid was developed in this research. The best fermentative conditions were determined finally. Among them, initial point for batch fermentation converting to extractive fermentation was 36h, dilution rate was 0.15h -1 , extractive fermentation period was 96h. Compared with conventional batch fermentation, this novel method increased both glucose conversion rate and L malic acid production rate evidently, decreased the fermentation period conspicuously.