miR-34b/c启动子区多态性与原发性肝细胞肝癌易感性的关联研究

A potentially functional polymorphism in the promoter region of miR-34b/c is associated with an increased risk for primary hepatocellular carcinoma

目的探讨miR-34b/c rs4938723 T>C与中国人群原发性肝细胞肝癌(hepatocellular carcinoma,HCC)易感性的关系。方法采用病例对照研究设计,选取501例原发性肝细胞肝癌病例和548例正常对照。选取miR-34b/c rs4938723 T>C为研究位点,以聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法进行多态性检测,应用Logistic回归计算OR值及95%CI,比较不同基因型与HCC发病风险的关系。结果在调整年龄、性别、吸烟、饮酒因素后,携带rs4938723 TC/CC基因型者与携带rs4938723 TT基因型个体相比,发生肝癌的风险增加了40%(调整OR=1.40,95%CI=1.10～1.80)。进一步分析显示在原发性肝细胞肝癌的发生中rs4938723基因型与饮酒存在交互作用(相乘交互作用P=0.049,相加交互作用P=0.012)。结论本研究表明位于miR-34b/c启动子区的rs4938723 T>C多态性可能影响中国人群原发性肝细胞肝癌发病风险。

Objective To investigate the associations between miR-34b/c rs4938723 T 〉 C and hepatocellular carcinoma （HCC） susceptibility in a Chinese population. Methods A case-control study among 501 HCC cases and 548 controls matched by age and sex was conducted, and the genotypes were determined by the polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） assays. Logistic regression analysis was used to evaluate the odd ratios （ORs） and 95% confidence intervals （CIs） of rs4938723 T〉C with susceptibility of HCC. Results The miR-34b/c rs4938723 TC/CC genotypes were associated with a significantly increased risk of HCC compared with their corresponding wild-type bomozygotes （adjusted OR = 1.40, 95 % CI = 1.10-1.80）. Furthermore, a significant interaction between alcohol drinking and rs4938723 T 〉 C on HCC risk was observed （P = 0. 049 for multiplicative and P = 0. 012 for additive interaction）. Conclusions These findings indicate that the potentially functional SNP rs4938723 in the promoter region of miR-34b/c are associated with the development of HCC in this Chinese population.