结直肠癌患者血清IL-23和IL-10水平的检测及其意义

Serum IL-23 and IL-10 assay in patients with colorectal carcinoma and significance in tumor progression

目的探讨结直肠癌进展过程中,患者血清中几种细胞因子水平的变化及其在肿瘤进程中的意义。方法纳入50例患者及20例健康志愿者,运用ELISA方法检测各组血清IL-23I、L-17I、L-10和IFN-α水平的变化。结果结直肠癌患者血清IL-23和IL-10水平显著高于对照组,而且Ⅳ期患者血清IL-10水平显著高于Ⅰ、Ⅱ、Ⅲ期。结论与IL-17和IFN-α相比,患者血清中IL-23和IL-10表达水平显著增加;此外,患者血清中IL-10的高水平表达可能促进了结直肠癌的发展。

Objective The aim of the study was to determine whether IL-23,IL-17,IL-10,and IFN-α levels are involved in the development and progression of colorectal carcinoma in humans.Methods A group of 50 patients and 20 healthy volunteers were included in the study.The quantitative determination of IL-23,IL-17,IL-10,and IFN-α serum levels were performed by ELISA.Results We observed significantly enhanced IL-23 and IL-10 in colorectal carcinoma patients compared to the controls.The highest level of IL-10 was detected in patients with stage IV,which was significantly greater than those in stages I,II,and III.Conclusion We found that IL-23 and IL-10 levels were significantly elevated in patients＇sera,in contrast to IL-17 and IFN-α and this serum cytokine profile may play a role in tumor development.In addition,an increased level of IL-10 was strongly associated with the progression of colorectal carcinoma.