胆囊腺癌pAKT、VEGF、微血管和微淋巴生成的检测及其临床意义

Significance of pAKT、VEGF、 Intratumour MVD and MLD in gallbladder carcinomas

目的研究胆囊腺癌组织中磷酸化丝氨酸/苏氨酸激酶(pAKT)、血管内皮生长因子(VEGF)蛋白的表达、微血管密度(MVD)和微淋巴管密度(MLD)及其临床意义。方法应用免疫组织化学SP法检测46例胆囊腺癌pAKT、VEGF、微血管和微淋巴管密度。结果胆囊腺癌pAKT和VEGF的阳性表达率分别为87.0%(40/46)和91.3%(42/46)。pAKT的阳性表达与临床分期和淋巴结转移显著相关,统计学有显著性差异(P<0.05)。MVD与肿瘤生长方式和临床分期显著相关;MLD与肿瘤生长方式和淋巴结转移有非常显著差异(P<0.01)。pAKT和VEGF阳性组的MVD明显高于其阴性组,Spearman相关分析表明pAKT和VEGF表达与MVD呈正相关。pAKT阳性组的MLD明显高于阴性组,Spearman相关分析揭示pAKT表达与MLD呈正相关,而VEGF阳性组的MLD与阴性组之间无显著性差异。结论 pAKT和VEGF与胆囊腺癌发生发展和转移密切相关,这提示阻断PI3K/AKT信号传导通路将对胆囊腺癌的治疗提供新的靶点。

Objective Methods Conclusion To study effect of expression of phosphorylated AKT（pAKT）,vascular endothyelial growth factor（VEGF） and on intratumour microvessel density（MVD）,microlymphatic density（MLD） in gallbladder carcinoma tissues.Methods Immunohistochemical method was used to detect the expressions of pAKT,VEGF,MVD,and MLD in 46 gallbladder carcinomas.Results The positive expression rates of pAKT and VEGF of gallbladder carcinoma were 87.0%（40/46）and 91.3%（42/46）respectively.The positive expression of pAKT was correlated to clinical staging and lymph node metastasis.The MVD was closely related to clinical staging and growth style.The MLD was associated with growth style and lymph node metastasis.The expression of pAKT and VEGF was positively correlation.Conclusion The results shown that pAKT and VEGF are might had important significance in the carcinogenesis,progression,and metastasis of gallbladder carcinoma,it might be as a potentially useful target for therapeutic intervention in gallbladder carcinoma patients.