MN基因定型在恶性血液病异基因造血干细胞移植植活证据检测中的临床研究

Graft survival assessment of MN genotype after allogeneic transplanttation of hematopoietic stem cell in Malignant haematology

目的探讨MN基因型定型在恶性血液病异基因造血干细胞移植植活证据检测中简单可行的方法。回顾性分析了10例患者恶性血液病异基因造血干细胞移植的临床资料。方法分别采取受者和供者移植前及移植后14天及365天的血液标本,根据已知基因序列设计合成引物,采取PCR-SSP的方法检测方法的检测受者和供者移植后的基因型和基因亚型。结果 10例患者MN为5例,MM为3例、NN为2例或MGMG2例,MTMT1例,MGMT6例,MTN1例。与血清学结果完全吻合。显示两组样本的表型与基因型吻合很好,资料的说明性较好(P>0.05)。结论血型基因检测的证据表明在同种异体造血干细胞移植中血型基因型可以作为早期监测的指标、标本用量少、科学敏感性强。

Objective study simple and feasible method of Graft survival assessment of MN genotype after allogeneic transplanttation of hematopoietic stem cell in Malignant haematology.Methods retrospective analysis the clinical data of 10 patients with malignant allogeneic blood hematopoietic stem cell transplantation,these recipients and donor were takeblood in transplantation 14 days before and after transplantation and 365 days later,according to known genes sequence design and synthesize two primer,detection genotype of receptor and donor recipients transplanted i n PCR-SSP method.Results 10patients were MN for 5 cases,MM for 3 cases,NN for 2 cases or MGMG for 2 cases,MTMT1 cases,MGMT6 cases,MTN1 cases.With serological results perfectly it show two groups of samples with genotype phenotype in good agreement with the prescriptive,material is good（P0.05）.Conclusion blood type gene detects the evidence in allogeneic blood hematopoietic stem cell transplantation in genotype can serve as early indicator,less specimen consumption and scientific sensitivity.