改良Giemsa法和硼酸美蓝法检测幽门螺杆菌感染的临床比较

Comparison of modified Giemsa(M-G) staining and boracic acid methylene blue(BAMB) staining on Helicobacter pylori detection

目的探讨改良Giemsa法和硼酸美蓝法在检测胃幽门螺杆菌(Hp)感染中的应用价值。方法收集80例慢性浅表性胃炎及胃溃疡患者,分别采用改良Giemsa法和硼酸美蓝法检测胃粘膜Hp,以Hp菌培养和C13呼气试验联合检测结果为Hp感染标准,比较两种检测方法对Hp检测的应用价值。结果以Hp菌培养和C13呼气试验联合检测结果为对照,改良Giemsa法检测Hp感染的特异性为100%,敏感性为96.2%(50/52),诊断符合率为97.5%(78/80);硼酸美蓝法检测Hp感染的特异性为100%,敏感性为88.5%(46/52),诊断符合率为92.5%(74/80)。两种方法检测Hp感染的特异性、敏感性及诊断符合率均较高,特异性和诊断符合率比较差异无显著意义(P>0.05),但改良Giemsa法的检测敏感性明显高于硼酸美蓝法(P<0.05)。结论改良Giemsa染色法和硼酸美蓝法具有较高的Hp感染检测特异性和诊断符合率,均为比较理想的常规Hp检测染色法。

Objective To investigate and compare modified Giemsa（M-G） staining and boracic acid methylene blue（BAMB） staining on Helicobacter pylori detection.Methods We enrolled 80 patients diagnosing as chronic superficial gastritis and gastric ulcer,then we used Modified Giemsa（M-G） staining and boracic acid methylene blue（BAMB） staining to evaluate HP infection of gastric mucosa and compared the positive detection rate of the above two methods.Results The sensitivity,specificity,and concordance rate of M-G was 100%,96.2%（50/52） and 97.5%（78/80）.Those of BAMB was 100%,88.5%（46/52） and 92.5%（74/80）.The specificity of HP with M-G was significantly higher than that with BAMB（P0.05）.There were not significantly difference in the sensitivity and concordance rate of M-G and BAMB（P0.05）.Conclusion M-G and BAMB are all the most sensitivity and differential method.But the specificity of HP with M-G is better than that with BAMB.