摘要
目的根据细菌16S rDNA的高度保守性,设计合成所有细菌的通用引物,建立快速检测细菌的方法。方法应用PCR方法检测细菌16S rDNA基因。同时对白假丝酵母菌、人白细胞DNA和HBV-DNA阳性病人血清同法检测。结果所有检测细菌均出现特异性条带,而白假丝酵母菌、人白细胞DNA和HBV-DNA阳性病人血清阴性。结论该法快速、特异、敏感。一个样品中能同时检测多种细菌,可为实验室初步判断是否存在细菌感染提供客观依据。
Objective According to the high conservative region of 16S rDNA gene in bacteria,PCR primers of the broad-range bceteria were synthesized to establish a method to rapidly detect bacteria.Methods Bacteria 16S rDNA gene were detected by PCR method.Candida albicans and human WBC DNA and the patient serum positive for HBV-DNA were detected by the same method simultaneously.Results The detected bactiria were all presented specific band,Candida albicans and human WBC DNA and the patient serum positive for HBV-DNA were all negative.Conclusion This protocol is rapid,specific,sensitive and capable of detecting multiple organisms in a single sample.This technology is of great utility for pilot laboratory judgement on the presence of bacteria.
出处
《中国实验诊断学》
北大核心
2011年第7期1140-1142,共3页
Chinese Journal of Laboratory Diagnosis
