摘要
目的研究老年性白内障晶状体上皮细胞中转化生长因子β2(TGF-β2)的表达变化情况。方法应用逆转录聚合酶链反应(semi-quantitaive reverse transcription polymerase chain reaction,RT-PCR)方法,检测TGF-β2在老年性白内障晶状体上皮细胞中mRNA的表达水平。结果老年性白内障晶状体上皮细胞中TGF-β2mRNA的表达水平高于对照组,差异有显著性。结论 TGF-β2的高表达提示其可能参与了老年性白内障晶状体上皮细胞的凋亡,在老年性白内障的发生、发展过程中起着重要的作用。
Objective To investigate the change of HSP27 and HSP70 expression of lens epithelial cells in age-related cataract.Methods RT-PCR method was used to study mRNA expression levels of TGF-β2 in lens epithelial cells of ARC.Results TGF-β2 mRNA＇s expression level is higher inARC than in control.Conclusion TGF-β2 is remarkable higher in ARC than in control group which suggests TGF-β2 plays an important role in the development of ARC.
出处
《中国实验诊断学》
北大核心
2011年第7期1148-1149,共2页
Chinese Journal of Laboratory Diagnosis
