摘要
建立气相色谱—质谱法测定食用植物油中反式脂肪酸的分析方法。食用植物油中的脂肪酸甲酯化后,经强极性毛细管柱SP—2560分离,采用质谱全扫描Scan方式得到各组分质谱图与标准谱库做相似度检索来定性,面积归一化法定量。用所建立的方法测定了6种常见食用植物油中反式脂肪酸含量,该法测定食用植物油中的反式脂肪酸含量前处理简单、快速,不需用标准品,分离良好,反式脂肪酸测定的相对偏差均小于8.5%,加标回收率在91.5%~98.5%之间,可用于实验室检测食用植物油中微量反式脂肪酸含量。
A method for the determination of trans-fatty acids in vegetable oil by GC—MS was developed.After methyl esterification,the fatty acid in vegetable oil was separated by SP—2560 column,qualified by GC—MS with Scan monitoring,determined by area normalization.The method has been successfully applied in determination of trans-fatty acids in 6 vegetable oils.The method was simple in pre-process,rapid,without standard sample.The RSD was within 8.5%,the recovery 91.5%~98.5%.It can be applied in the lab.
出处
《粮油食品科技》
2011年第4期20-22,共3页
Science and Technology of Cereals,Oils and Foods
关键词
食用植物油
反式脂肪酸
气相色谱—质谱法
edible vegetable oil
trans fatty acid
gas chromatography—mass spectrometry