bcl-2基因在成年和胚胎鸡免疫器官中的表达及其与细胞凋亡的关系

Relationship between bcl-2 expression and apoptosis in chicken immune organs including adult and embryo

用一步法从17 日龄来克亨SPF鸡胚和90 日龄成年鸡的法氏囊、脾脏和胸腺组织中提取总RNA(TRNA),用鸡B-细胞淋巴瘤/白血病-2基因 (bcl-2) cDNA探针进行Northern blot杂交。发现bcl-2 在成年和胚胎鸡的不同免疫器官中表达量不同, 成年鸡bcl-2 的表达量多少依次为胸腺、脾脏、法氏囊, 而胚胎鸡则是胸腺、法氏囊、脾脏。同时用流式细胞法测定这些器官的细胞凋亡情况, 胚胎鸡法氏囊、脾脏和胸腺的细胞凋亡率分别是0.40% , 0.68% 和0.38% ; 成年鸡则是6.98% , 1.17% 和0.61% 。结果表明, 鸡bcl-2 基因通过阻抑细胞凋亡直接调控正常免疫器官的发育, 在淋巴细胞发育过程中bcl-2是免疫细胞亚型选择的重要调节物。

Total RNA was extracted with single step method in bursa,spleen and thymus including 17 day old embryo and 90 day old adult from SPF White Leghorn chickens,and was hybridized with a chicken bcl 2 cDNA probe.It is found that bcl 2 expression was different in chicken immune organs.In embryo chicken,the bcl 2 gene was expressed in the immune organs,with the highest levels of the bcl 2 transcripts being detected in thymus,lower in bursa,and the lowest in the spleen.On the other hand,the gene was detected in adult′s thymus and spleen,the buras expressed much less amounts of bcl 2 RNA.The apoptotic rate in the organs was determined with flow cytometry.The results showed that the value is 0 40%,0 68% and 0 38% in bursa,spleen and thymus from embyro chicken,and in adult,the apoptotic rate in bursa,spleen and thymus is 6 98%,1 17% and 0 61%, respectively.It is indicated that bcl 2 gene regulates and controls directly apoptosis in chicken immune organs,and appears to be a critical regulator in selection of subset of immune cells. ABSTRACT Total RNA was extracted with single step method in bursa,spleen and thymus including 17 day old embryo and 90 day old adult from SPF White Leghorn chickens,and was hybridized with a chicken bcl 2 cDNA probe.It is found that bcl 2 expression was different in chicken immune organs.In embryo chicken,the bcl 2 gene was expressed in the immune organs,with the highest levels of the bcl 2 transcripts being detected in thymus,lower in bursa,and the lowest in the spleen.On the other hand,the gene was detected in adult′s thymus and spleen,the buras expressed much less amounts of bcl 2 RNA.The apoptotic rate in the organs was determined with flow cytometry.The results showed that the value is 0 40%,0 68% and 0 38% in bursa,spleen and thymus from embyro chicken,and in adult,the apoptotic rate in bursa,spleen and thymus is 6 98%,1 17% and 0 61%, respectively.It is indicated that bcl 2 gene regulates and controls directly apoptosis in chicken immune organs,and appears to be a critical regulator in selection of subset of immune cells. Total RNA was extracted with single step method in bursa,spleen and thymus including 17 day old embryo and 90 day old adult from SPF White Leghorn chickens,and was hybridized with a chicken bcl 2 cDNA probe.It is found that bcl 2 expression was different in chicken immune organs.In embryo chicken,the bcl 2 gene was expressed in the immune organs,with the highest levels of the bcl 2 transcripts being detected in thymus,lower in bursa,and the lowest in the spleen.On the other hand,the gene was detected in adult′s thymus and spleen,the buras expressed much less amounts of bcl 2 RNA.The apoptotic rate in the organs was determined with flow cytometry.The results showed that the value is 0 40%,0 68% and 0 38% in bursa,spleen and thymus from embyro chicken,and in adult,the apoptotic rate in bursa,spleen and thymus is 6 98%,1 17% and 0 61%, respectively.It is indicated that bcl 2 gene regulates and controls directly apoptosis in chicken immune organs,and appears to be a critical regulator in selection of subset of immune cells.