转DREB1A基因地被菊耐旱节水性

Drought Tolerance and Water Saving Ability in DREB1A Transgenic Plants of Ground Cover Chrysanthemum ‘Fall Color'

选用已经获得的具有多重抗逆性的转DREB1A基因地被菊6个株系为试验材料,以未转基因的野生型植株‘WT’为对照,将各株系盆栽幼苗分别进行室温条件下和高温条件下的水分胁迫处理,通过研究水分胁迫前后各株系植株生理指标的变化规律,分析了各株系的耐失水特性,并利用方差分析及隶属函数综合评价的方法对不同株系的耐旱节水能力进行了评价。结果表明,在6个转基因地被菊株系中有5个株系的耐旱节水性优于‘WT’,且其中3个株系与‘WT’差异显著(p<0.05);有1个株系耐旱节水性不如‘WT’,但与‘WT’差异不显著。

Six transgenic lines of ground cover chrysanthemum over-expressing AtDREB1A that conferred strong tolerance to high temperature and drought stress were treated for dehydration at different temperature conditions, using ＇ Wild Type＇ as the control group. The drought resistance of different chrysanthemum lines was studied by measuring physiological indices related with drought tolerance. A comprehensive evaluation on drought resistance and water saving ability of different lines were made according to the variance analysis and the subordinate function values. Results indicate that, five transgenic lines are better than the control plants, and there is a significant difference （p〈0. 05） in drought tolerance and water saving ability between three of them and the control. One transgenic line is not as good as the control, but there is no significant difference between them.