迷迭香酸对大鼠肾草酸钙结石形成的影响

Effect of rosmarinic acid on renal calcium oxalate stone formation in rats

目的:探讨迷迭香酸对大鼠肾草酸钙结石形成的影响。方法:Wistar♂大鼠36只,随机分为6组,每组6只,分别为正常组,模型对照组,不同剂量迷迭香酸组和阳性对照组(肾石通颗粒剂)。采用1%乙二醇+2%氯化铵,每天ig一次诱导大鼠草酸钙结石模型形成,连续28d,各组同步给药或纯化水。检测各组大鼠成石不同时期的尿液肌酐(Cr)、尿酸(UA)水平,草酸(OX)、枸橼酸(Cit)和Ca2+含量。造模28d结束后,检测血生化及肾草酸(OX)、枸橼酸(Cit)和Ca2+含量,测定肾指数,采用HE和Von-Kossa’s染色,观察肾组织病理变化及草酸钙结晶发生与形态。结果:迷迭香酸中剂量(0.05g.kg-1)、高剂量(0.1g.kg-1)组大鼠24h尿Cr、UA的水平及尿OX、Ca2+的排泄量,血清Cr、BUN、UA,肾组织OX、Ca2+含量,均显著低于模型对照组;肾组织病理变化明显改善,草酸钙的结晶发生明显少于模型对照组,形态分布程度也减轻,而肾指数增加。结论:迷迭香酸可以抑制大鼠草酸钙结石的形成。

OBJECTIVE To investigate the effect of rosmarinic acid on the calcium oxalate renal stone formation in rats. METHODS Thirty-six Wistar ♂ rats were randomized into six groups： normal control group, model group, rosmarinic acid low-dose group, middle-dose group, high-dose group, positive control group （Shenshitong Granule）. The rat model of renal calcium oxalate stones was induced by ig administration of 1 % ethylene glycol and 2% ammonium chloride for 28 days. In addi tion to renal stone formation treatment, six groups of rats were also given different test drugs or distilled water. The contents of urine creatinine, uric acid, oxalic acid, citric acid, Ca^2＋ were detected during different times. After 28 days, the serum bio chemical indices, renal tissue oxalic acid and citric acid, renal tissue Ca^2＋ and renal index were detected and renal pathology was examined. RESULTS The 24 h urinary excretions of creatinine, uric acid, Ca^2＋ , oxalic acid, as well as the serum creatinine, uric acid and blood urea nitrogen levels, the contents of renal tissue oxalic acid and the calcium in kidney, were significantly lower in middle-dose （0.05 g·kg^-1） and high-dose （0. 1g·kg^-1） groups than those in model group （P〈0. 05）. The calcium oxalate deposits in the kidney and the pathological change degrees of the kidneys were decreased and kidney index was higher in middle-dose group and high-dose group than those in model group. CONCLUSION Rosmarinic acid can inhibit renal calcium oxalate stone formation in rats.