寒热环境刺激对DEN诱发性肝癌大鼠Th1和Th2细胞因子的影响

Effect of Cold and Heat Environment Stimulation on Th1/Th2 Cytokines of Diethylnitrosamine-Induced Liver Cancer in Rats

目的:研究寒热环境刺激对二乙基亚硝胺(diethylnitrosamine,DEN)诱发性肝癌大鼠模型血清Th1、Th2细胞因子的影响。方法:通过灌胃DEN(按体质量10mg/kg给予0.2%DEN灌胃,每日1次,5次/周,连续14周)建立诱发大鼠肝癌模型,同时给予特定的寒热环境刺激,每天连续3h,5次/周,至20周末处死所有大鼠,HE染色观察大鼠肝脏组织学变化;计算大鼠的脾体比、成瘤率、死亡率;ELISA检测大鼠血清IL-2、IFN-γ、IL-10、IL-4的含量。结果:与模型组比较,热环境组成瘤率、死亡率、脾体比相对较高,IL-2、IFN-γ浓度降低,IL-10、IL-4浓度升高;而寒环境组则与之相反,且寒热两组间差异显著(P<0.05)。结论:长期的寒热环境刺激可对DEN诱发性肝癌大鼠模型的肿瘤生成和预后呈现不同影响,而引起Th1和Th2细胞因子的改变可能是其主要的作用机制之一。

Objective：To study the effect of cold and heat enviroment stimulation on Th1/Th2 cytokines of diethylnitrosamine（DEN）-induced liver cancer in rats.Methods：The liver cancer model of rats was established by intragastric administration of DEN（a dosage of 10mg/kg,once a day,five times a week for 14 weeks）.These rats received cold and heat stimulation respectively lasted for 3 hours every day for 20 weeks.Hematoxylin and eosin（HE） staining was used to examine the changes of liver pathology.The mortality,tumor-formation rate and the ratio of spleen and weight were measured and the concentration of IL-2、IFN-γ、IL-10、IL-4 in blood serum of rats were detected by ELISA.Results：Compared with model group,the mortality,tumor-formation rate,the ratio of spleen and weight and the concentration of IL-10、IL-4 were increased,the concentration of IL-2、IFN-γwere decreased in heat stimulation group,while the opposite results were observed in cold stimulation group.And the difference of the two groups is significant.Conclusion：Some impart of long-term cold and heat stimulation on formation and prognosis of tumor can be observed in DEN-induced rat model of liver tumor.Causeing the change of concentration of Th1/Th2 cytokines may be one of the mechanisms.