陆地棉体胚发生及再生植株移栽

SOMATIC EMBRYOGENESIS AND REGENERATED PLANT TRANSPLANTATION IN UPLAND COTTON (GOSSYPIUM HIRSUTUM L.)

以体细胞培养难易不同的Corker312，泗棉3号，川棉239为材料，比较研究了它们在不同培养基上愈伤组织诱导和胚性愈伤组织发生的差异，获得了3个品种的再生植株，并对体胚苗移栽适宜基质进行了研究。

Three upland cotton varieties, i. e, Corker 312 and two varieties from the cotton-growing regions of the Yangtze River Basin,were cultured on four different media. On MSB medium containing 2,4-D 0.05 mg/L+ KT 0.1mg/L or NAA 4.0mg/L+ KT 1 .0 mg/L, the the of callus inducing was very high for all the varieties studied. While the rate of callus induction was high for Corker 312 on the medium with IAA 0.5 mg/L+ KT0.1 mg/L or ZT 0.1 mg/L, it was very fow for the other two varieties. Corker 312 produced somatic embryos on all the media used in this study, while the two varieties from the Yangtze River Basin regenerated embryos only on the medium with IAA or ZT.Planilets were obtained for all the three varieties.The media suitable for transplanting the somatic embroy-derived plantlets were investigated.