氟苯尼考在鲟鱼体内的药物代谢动力学研究

Pharmacokinetics of a Chemical Florfenicol in Sturgeon

采用高效液相色谱检测组织中药物含量,研究水温在(20±1)℃时,杂交鲟经口一次性给予10 mg/kg氟苯尼考后在血液、肌肉、肝脏中的代谢规律。试验结果表明,氟苯尼考经口给药后在鲟鱼血液中的代谢属于一级吸收一室模型,在肌肉和肝脏中属于一级吸收二室模型,且分布广泛,代谢迅速。主要药代动力学参数如下:血液、肌肉、肝脏中的达峰质量浓度和达峰时间分别为6.43、5.81、6.062μg/ml和9.84、8.88、0.51 h;吸收半衰期分别为4.29、4.61、0.27 h;消除半衰期分别为9.45、21.69、11.04 h;药时曲线面积分别为169.02、227.41、50.56 mg/(L.h);表观分布容积分别为0.81、1.07、0.80 L/kg;清除率分别为0.059、0.051、0.2 L/kg。方法回收率≥90%,线性相关系数r2=0.9996。

The pharmacokinetics of florfenicol were studied in blood,muscle and liver in hybrid（Acipenser schrenckii×Huso dauricus） sturgeon sampled after intravenous injection at single dose of 10 mg/kg body weight by HPLC,and the data were analyzed by pharmacokinetics computer program 3P97.The results showed that the concentration-time course of the florfenicol was described by one-compartment model with 1st order absorption in the blood and two-compartment model with 1st order absorption in the muscle and liver,with wide distribution and rapid metabolism.The main pharmacokinetics parameters in blood,muscle and liver were found as following：Cmax 6.43,5.81,and 6.062 μg/ml;Tmax 9.84,8.88,and 0.51 h;t1/2α29,4.61,and 0.27 h;t1/2β 9.45,21.69,and 11.04 h;AUC 169.02,227.41,and 50.56 mg/（L·h）;V/f 0.81,1.07,and 0.80;CL/f（s） 0.059,0.051,and 0.2 L/kg;recovery≥90%,R2=0.9996.