稻白叶枯病菌对水稻悬浮细胞H_2O_2含量及其代谢酶活性的影响

Effects of Xanthomonas oryzae pv.oryzae on Hydrogen Peroxide Contents and Its Metabolizing Enzymes in the Suspension Cells of Rice

为探讨H2O2及其代谢酶与水稻抗白叶枯病的关系,以水稻品种余水糯和浙辐802的悬浮细胞为材料,研究了稻白叶枯病菌(751和7625菌株)对水稻悬浮细胞中H2O2含量,CAT,POD和SOD活性的影响.结果表明:白叶枯病菌接种水稻品种余水糯悬浮细胞,其H2O2含量增加,而CAT,POD和SOD活性均低于对照.白叶枯病菌侵染对水稻品种浙辐802悬浮细胞中H2O2含量,CAT,POD和SOD活性影响不大.由此可见,H2O2含量,CAT,POD和SOD活性与水稻白叶枯病抗性有密切关系.

The changes in H 2O 2 contents,activities of CAT,POD and SOD were investigated in suspension cells of rice infected by Xanthomonas oryzae pv.oryzae (strain 75 1 and 76 25) by using rice Yushuinuo and Zhefu 802 with different resistances to Xanthomonas oryzae pv.oryzae as the experimental materials. The results showed that the differences in H 2O 2 contents and its metabolizing enzymes were found in the suspension cells of Yushuinuo and Zhefu 802. H 2O 2 contents in the suspension cells of Yushuinuo with high resistance to Xanthomonas oryzae pv.oryzae after inoculation with Xanthomonas oryzae pv.oryzae increased,while,compared with those of the control,activities of CAT,POD and SOD decreasedin thesuspensioncellsof Yushuinuo afterinculationwith Xanthomonas oryzae pv. oryzae.  The effects of Xanthomonas oryzae pv.oryzae on H 2O 2 contents,activities of CAT,POD and SODin the suspension cells of Zhefu 802 withsusceptibility to Xanthomonas oryzae pv.oryzae was slight.We suggest that the resistance to Xanthomonas oryzae pv.oryzae in rice be correlated with accumulation of H 2O 2 and decrease of activities of CAT,POD and SOD.