益气温阳活血方对早期心力衰竭大鼠血管紧张素Ⅱ和醛固酮的影响

Effects of decoction of yiqiwenyanghuoxue on Ang Ⅱ and ALD in rats with early heart failure

目的探讨中药复方益气温阳活血方对早期心力衰竭大鼠血管紧张素Ⅱ(AngⅡ)、醛固酮(ALD)的作用机制。方法以阿霉素尾静脉注射造模;超声检测评价其心功能、应用放射免疫法检测AngⅡ、ALD浓度、以RT-PCR法及W estern印迹法检测过氧化物酶体增殖物激活受体γ(PPARγ)mRNA及蛋白质表达水平在益气温阳活血方治疗前后的变化。结果随着心力衰竭的加重、心室重构的形成,模型大鼠AngⅡ、ALD含量进行性升高,而PPARγmRNA与蛋白质的表达水平则进性降低。益气温阳活血方在心力衰竭的早期即可减少AngⅡ、ALD含量及上调PPARγmR-NA与蛋白质的表达水平。结论 AngⅡ、ALD可作为早期心力衰竭预警标志物;益气温阳活血方在心力衰竭早期可通过PPARγ信号通路调节AngⅡ、ALD的表达,防止心室重塑,改善心脏功能。

Objective To investigate the effects of decoction of yiqiwenyanghuoxue on Ang Ⅱ and aldosterone（ALD） in rats with early heart failure.Methods Model rats with chronic heart failure（CHF）were injected with Adriamycin and treated by decoction of yiqiwenyanghuoxue,then AngⅡ and ALD levels were detected by radioimmunity.PPARγ mRNA were detected by RT-PCR and protein by Western-blot.Results Compared to the control group,Ang Ⅱ and ALD levels were significantly higher,but PPARγ at mRNA and protein levels were significantly lower in CHF groups.Yiqiwenyanghuoxue decoction could improve the above changes.Conclusions AngⅡ,ALD can be the predictors in early heart failure.Decoction of yiqiwenyanghuoxue can prevent myocardial remodeling and improve heart function through PPARγ regulating AngⅡand ALD levels.