ICU多重耐药鲍曼不动杆菌的耐药性和同源性分析

Multidrug Resistance of Acinetobacter Baumannii and Homology Analysis

目的通过对重症监护病房(ICU)的多重耐药鲍曼不动杆菌进行同源性分析,进行院内感染的基线调查,并分析其耐药谱特征,为临床防治和控制院内感染提供依据。方法测定17种抗菌药物对62株鲍曼不动杆菌的最低抑菌浓度(M IC),并采用随机扩增多态性DNA(RAPD)技术分析其耐药株的同源性。结果有16株鲍曼不动杆菌对头孢菌素类、氨基甙类和氟喹若酮类等抗菌素均显示出较高水平的多重耐药性;其中4株RAPD分型显示有高度同源性。结论分离自ICU的鲍曼不动杆菌多重耐药率高达21%,且大多数菌株有高度同源性。因此建立全面的药敏谱和RAPD基因分型体系,加强鲍曼不动杆菌耐药监测,为及时找到感染源头、有效控制医院感染提供依据。

Objective Through the homogeneous analysis of the muhidrug resistant Acinetobacter baumannii in the intensive care unit （ICU） ,the baseline survey of hospital infection was carried out and the spectral characteristics of drug resistance was analyzed so as to provide evidence for clinical prevention and control of hospital infection. Methods The minimum inhibitory concentration （MIC） of 17 kinds of antimicrobial agents against 62 strains of Acinetobacter baumannii were detected and the homology of resistant strains were analysed by using random amplified polymorphic DNA （RAPD） technology. Results Sixteen strains of Aeinetobacter baumannii were multi-resistant to eephalosporins, aminoglycosides and fluoroquinolone antibiotics, 4 strains were highly homologous by randomly amplified polymorphie DNA technique （RAPD）. Conclusion The multi-drug resistance rate of Aeinetobacter banmannii isolated from ICU was up to 21%. Most of the strains were highly homologous by RAPD technique. In order to find the source of infection and provide evidence of controlling hospital infections effectively, we should establish a comprehensive spectrum of antibiotics and RAPD genotyping system and strengthen the monitoring of drug resistance of Acinetobacter baumannii.