Jagged 1在生理性和病理性新生血管形成中的表达变化

Study of the role of Jagged 1 in physiological and pathological neovascularization

目的观察Jagged 1在生理性和病理性新生血管形成中的表达变化,为肿瘤的治疗寻找新的靶点。方法体外培养血管内皮细胞免疫细胞化学法检测其Jagged 1的表达。建立鸡胚尿囊膜动态血管发育模型,免疫荧光化学和Westernblot检测不同发育时期尿囊膜血管中Jagged 1的表达。免疫组织化学和Western blot检测正常结直肠组织和大、中、小三种尺寸的结直肠癌肿块中Jagged 1的表达。结果 (1)免疫细胞化学结果显示,Jagged 1是细胞跨膜蛋白,表达于细胞膜上。(2)成功建立鸡胚尿囊膜动态血管发育模型,其免疫荧光和Western blot结果显示,Jagged 1随着血管的发育而表达,随血管密度、血流量增加而表达上调。(3)免疫组化结果显示Jagged 1在结直肠癌中广泛表达,而在正常皮肤及结直肠组织中表达较少,Jagged 1的表达量同肿瘤的体积有关。结论 Jagged 1在内皮细胞、血管发育初期和肿瘤新生血管中表达上调,表明其可能是新生血管形成的早期事件,参与诱导下游新生血管形成基因的表达。

Objective To research the role of Jagged 1 in physiological and pathological neovascularization. Methods The expression of Jagged 1 were detected in endothelial cells by immunocytochemistry.The expression of Jagged 1 were detected in blood vessel of chick chorioallantoic membrane by immunofluorescence and Western blot.The expression of Jagged 1 were detected in normal colorectal mucosa tissue and three colorectal cancers with large,median and small volume by immunohistochemistry and Western blot. Results（ 1）Results of immunocytochemistry showed that Jagged 1 is a cell transmembrane protein and express in the cell membrane.（ 2）Successfully established the dynamic blood vessel developmental model of chick chorioallantoic membrane, The dynamic blood vessel developmental model of chick chorioallantoic membrane showed that the vascular network in chorioallantoic membrane develops gradually with embryo age and reveals the process of neovascularization, vigorous growth and wilt dynamically. Results of immunofluorescence and Western blot revealed that Jagged 1 has different expression profiling at different period of vascular development.The expression of Jagged 1 increased with the increasing of physiologic vessels,vessel density,blood supply.（ 3）Results of immunohistochemistry performed on hemangioma and colorectal cancer,Jagged 1 was widely expressed in neonatal vessels originated from hemangioma proliferation and few expressed in normal skin tissue.The expression of Jagged 1 was related to the tumor size in colorectal cancer. Conclusion The expression of Jagged 1 up-regulated in endothelial cells,primary period of vascular development and tumor angiogenesis,which may be an early event in neovascularization involved in inducing the expression of downstream gene.