CYP1A1基因I462V多态与非小细胞肺癌的相关性

Association of I462V polymorphism of CYP1A1 gene with non-small cell lung cancer

目的探讨CYP1A1基因I462V多态性与非小细胞肺癌的相关性。方法采用病例对照研究,应用聚合酶链反应-限制性片段长度多态性对72例非小细胞肺癌患者(病例组)和90例正常对照(对照组)CYP1A1基因I462V多态进行检测,分析基因型频率和等位基因频率在病例组和对照组的分布,比较不同基因型与非小细胞肺癌患病风险的关系。应用SPSS13.0进行统计学分析。结果在非小细胞肺癌组和正常对照组I462V多态基因型频率(2=14.971,P=0.001)和等位基因频率(2=9.506,P=0.002)的分布存在显著性差异,且G等位基因携带者患非小细胞肺癌的风险高于A等位基因携带者(OR=2.080,95%CI1.301～3.324)。结论 CYP1A1基因I462V多态与非小细胞肺癌具有明显的相关性,具有G等位基因的个体非小细胞肺癌患病风险增高,CYP1A1基因可能是非小细胞肺癌的遗传易感基因。

Objective To investigate the association of I462V polymorphism of CYP1A1 gene with non-small cell lung cancer （NSCLC）.Methods Under the case-control study,the I462V polymorphisms of CYP1A1 gene in 72 patients with NSCLC and 90 healthy controls were detected with polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）.The distribution of genotype and allele frequency at the I462V polymorphism and its relationship with the risk of NSCLC were analyzed in these two groups.SPSS13.0 software was used for the data analysis.Results The distribution of genotype at I462V polymorphism site was significantly different between NSCLC group and healthy control group （2=14.971,P=0.001）,the distribution of allele frequency at I462V polymorphism site was also significantly different between two groups （2=9.506,P=0.002）.The relative risk for T NSCLC in G allele carriers was higher than that in A allele carriers （OR=2.080,95%CI 1.301～3.324）.Conclusions The 462V polymorphism of CYP1A1 gene is associated with NSCLC,with much higher risk for G allele,CYP1A1 gene may be a genetic susceptibility gene of NSCLC.