摘要
目的探讨CD8＋CXC趋化因子受体3（CXCR3）＋调节性T细胞在自身免疫性溶血性贫血（AIHA）发病中的作用。方法用流式细胞术检测2010年7—11月天津医科大学总医院血液科收治的11例初治（初治组）、11例治疗后完全缓解（完全缓解组）AIHA患者的外周血CD8＋CXCR3＋／CD8＋T细胞比例及CD8＋CXCR3＋白细胞介素10（IL-10）＋／CXCR3＋CD8＋T细胞比例，并与23名健康对照（对照组）比较。分析上述指标与CD3＋CD4＋细胞数量及CD5＋CD19＋／CD19＋比值的相关性。用反转录-聚合酶链式反应（RT-PCR）技术检测外周血单个核细胞（PBMC）中CXCR3mRNA的表达水平。结果AIHA初治组外周血CD8＋CXCR3＋／CD8＋为（39．80±19．96）％，低于完全缓解组[（58．76±14．22）％，P〈0．05]及对照组[（59．66±12．62）％，P〈0．01]。初治组外周血CD8＋CXCR3＋IL-10＋／CXCR3＋CD8＋为（22．98±14．96）％，低于对照组[（38．15±17．03）％，P〈0．05]。初治组PBMCCXCR3mRNA相对表达量为（0．51-±0．19），低于对照组（1．67±1．17，P〈0．01）。外周血CD8＋CXCR3＋／CD8＋与CD3＋CD4＋细胞数量及CD5＋CD19＋／CD19＋比值均呈负相关（r=-0．571，-0．583；均P〈0．05）；CD8＋CXCR3＋IL-10＋／CD8＋CXCR3＋与CD3＋CD4＋细胞数量及CD5＋CD19＋／CD19＋比值均呈负相关（r=-0．524，-0．523；均P〈0．05）。结论CD8＋CXCR3＋调节性T细胞数量减少、产生IL-10水平下降可能导致AIHA患者免疫耐受被打破，与AIHA的发病有关。
Objective ＂To explore the effects of CD8 ＋ CXCR3 ＋ T cells on autoimmune hemolytic anemia （AIHA）. Methods Twenty-two AIHA patients, including 11 untreated and 11 recovered ones, and 23 normal controls were recruited from July 2010 to November 2010. The percentage of CD8 ＋ CXCR3 ＋ / CD8 ＋ T cells in peripheral blood and the expression of interleukin-10 （IL-10） in CD8 ＋ CXCR3 ＋ T cells were detected by flow cytometry. Their correlations with the count of CD3 ＋ CD4 ＋ cells and the percentage of CD5＋ CD19 ＋ in CD19＋ B cells were analyzed. The expression level of CXCR3 mRNA in PBMC was determined by semi-quantitative reverse transcription-polymerase chain reaction （RT-PCR）. Results The percentage of CD8＋ CXCR3 ＋/CD8 ＋of untreated AIHA patients was （39. 80 ±19.96）%. And it was lower than that of recovered patients [ （58.76 ± 14. 22） %, P 〈 0. 05 ] and normal controls [ （59.66 ± 12. 62） %, P 〈 0. 011. The percentage of IL-10 ± T cells in CD8 ＋ CXCR3 ＋T cells of untreated patients was （ 22. 98 ±14. 96）% and it was lower than that of normal controls [ （38. 15 ±17.03）% ,P 〈0. 05]. The expression level of CXCR3 mRNA for untreated AIHA patients was （0. 51 ±0. 19） and it was lower than that of normal controls （1.67± 1.17, P 〈 0. 01 ）. The percentage of CD8＋ CXCR3＋/CD8＋ T cells had a negative correlation with the count of CD3 ＋ CD4 ＋ cells and the percentage of CD5 ＋ CD19 ＋/CD19＋ B cells （r = - 0. 571, - 0. 583,both P 〈 0. 05）. So did the percentage of IL-10 ＋ T cells in CD8 ＋ CXCR3 ＋ T cells （r = - 0. 524, - 0. 523, both P 〈 0. 05）. Conclusion The decreased count of CD8 ＋CXCR3 ＋T cells and the lowered level of IL-10 may disturb the immune tolerance and lead to the occurrence of AIHA.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2011年第24期1687-1690,共4页
National Medical Journal of China
基金
国家自然科学基金（30971285、30971286）
卫生部卫生行业科研专项（201002024）
高等学校博士学科点专项科研基金（200800620004）
天津市应用基础及前沿技术研究计划（08JCYBJC07800、09JCYBJC11200）
天津市卫生局科技基金（09KZ108、07KZ20、11KG135）
