补肾健脑方对血管性痴呆大鼠皮质和海马脑血流量及乙酰胆碱含量的影响

Effects of Bushen Jiannao Decoction on cerebral blood flow and content of acetylcholine in cerebral cortex and hippocampus in vascular dementia rats

目的研究补肾健脑方对血管性痴呆(VD)大鼠学习记忆、局部脑血流量、乙酰胆碱(ACh)含量及CA1区神经元形态学改变的影响,探讨其治疗VD的可能机制。方法双侧颈总动脉永久结扎法(2-VO)制备大鼠慢性脑缺血致VD动物模型,造模完成后,随机分为6组。分别用蒸馏水、多奈哌齐混悬液以及不同浓度补肾健脑方灌胃,30 d后水迷宫实验测定大鼠学习记忆能力,放射性测量脑血流量,比色法测定ACh含量,HE染色观察神经元形态学改变。结果与模型组相比,补肾健脑方能提高VD大鼠的学习记忆能力,改善皮质和海马脑血流量,提高ACh含量,与多奈哌齐组相比未见统计学差异。结论补肾健脑方改善VD大鼠的学习记忆能力的作用机制可能是通过增加皮质和海马的脑血流量,增加皮质和海马ACh含量而发挥作用的。

Objective To observe the effects of Bushen Jiannao Decoction on learning and memory, regional cerebral blood flow, and content of acetylcholine in cortex and hippocampus in vascular dementia （VD） rats so as to explore the drug＇s possible mechanisms for treating VD. Methods VD models were established by permanent bilateral occlusion of both common carotid arteries and were then divided randomly into six groups. Distilled water, donepezil and Bushen Jiannao Decoction of different concentrations were used in gastric lavage once a day. After 30 days, Morris water maze experiment was performed to test the rats＇ learning and memory. Then cerebral blood flow was determined radiologically, content of acetylcholine in the hippocampus and cortex was assayed with colorimetry, and morphologic changes of neurons were observed with HE staining. Results Compared with those in model group, learning and memory were highly improved, and cortical cerebral blood flow and content of acetylcholine in the cortex and hippocampus significantly increased in VD rats of Bushen Jiannao Decoction group. However, no significant difference was found between Bushen Jiannao Decoction group and donepezil group. Conclusion The possible mechanisms of Bushen Jiannao Decoction for treating vascular dementia may be increasing acetylcholinc level in the cortex and hippocampus through improving cerebral blood flow in the cortex and hippocampus.