血栓通注射液联合贝那普利对糖尿病肾病大鼠肾脏病理学及h-CRP、TNF-α表达的影响

Effects of Xueshuantong injection combined with benazepril on renal pathological changes of diabetic nephropathy and on h-CRP and TNF-α expression

目的观察血栓通注射液联合贝那普利对糖尿病肾病(DN)大鼠肾脏的保护作用,探讨其作用机制。方法用高糖高脂饲料加小剂量STZ制备DN大鼠模型,随机分为模型组(DN组)、贝那普利组(B组)、贝那普利加血栓通组(X组)、空白组(N组)。于实验12周末检测超敏C反应蛋白(h-CRP)、肿瘤坏死因子a(TNF-a),观察肾组织病理改变。结果与DN组相比,X组TNF-α、h-CRP水平明显降低(P<0.01),肾小球球囊增大程度减轻,基底膜增厚有所减轻,肾小球系膜扩张程度下降。与B组相比,X组TNF-α、h-CRP水平降低更显著(P<0.05),肾小球基底膜增厚及系膜细胞增生病变明显改善。结论血栓通联合贝那普利对DN大鼠的肾脏有明显的保护作用,其机制可能与降低炎性因子h-CRP、TNF-α的表达及抑制DN大鼠肾小球系膜基质增生及细胞外基质的沉积有关。

Objective To observe the protective effects on diabetic nephropathy（DN） rats by Xueshuantong injection combined with benazepril and to explore its therapeutic mechanism.Methods DN rat models were prepared by high-glucose and high-fat forage added with small-dose STZ.The rats were randomized into DN group without intervention（DN group）,DN with benazepril group（B group）,DN with benazepril and Xueshuantong injection group（X group） and normal group（N group）.The level of h-CRP and TNF-α expression was measured at the end of 12th week.The renal pathomorphologic changes were observed.Results The level of h-CRP and TNF-α expression in X group obviously decreased as compared with that in DN group（P〈0.01）.The level of h-CRP and TNF-2 expression in X group significantly decrensed as comparerd with that in B group.Gromeralor basement membrane thickening and mesangial cell proliferative lesions were significantly improved.Conclusion Xueshuantong injection combined with benazepril can significantly protect DN rat’s kidney,which might be its mechanism of reducing h-CRP and TNF-α expression and inhibiting the DN proliferation of mesangial matrix and deposition of extracellular matrix.