不同年龄实验恒河猴自然死亡的肝脏病变谱分析

Analysis of liver pathology in captive rhesus monkeys at different ages

目的观察人工饲养条件下实验恒河猴肝脏病理改变,探讨肝脏疾病分布规律和病理改变特点,丰富实验猴自发病变基本研究资料。方法对1998～2008年云南地区饲养的自然死亡的155只恒河猴(年龄2～20岁)的肝脏进行病理检查,按年龄分为幼年组、成年组、老年组,并对观察结果进行统计学分析。结果 155例恒河猴中88例检出肝脏病变,有肝细胞变性、肝细胞坏死、炎细胞浸润、吞噬细胞增生、肝淤血、纤维组织增生、肝脓肿、寄生虫共八种主要病变,出现率最高的为肝细胞水样变性(34.19%)。除肝脓肿外,幼年组、成年组、老年组八种病变均有检出。卡方检验显示:肝细胞水样变性成年组病变率明显高于幼年组;肝细胞脂肪变性老年组明显高于成年组和幼年组;轻度炎细胞浸润病变老年组明显高于成年组;纤维组织增生老年组明显高于幼年组(P<0.05)。结论人工饲养条件下死亡实验猴肝脏病变检出率较高,实验猴肝脏病理改变随年龄增长而病变加重,提示在进行实验猴肝脏研究时,应注意对自发性病变的判别,药物安全性评价实验应避免选择老年猴做为研究对象。死亡实验猴肝脏病变谱研究,对实验猴的质量控制和相关动物实验有重要指导价值。

Objective To observe the pathological changes in the livers of captive rhesus monkeys,and explore their distribution rule and provide basic background reference data for relevant biomedical studies.Methods One hundred and fifty five natularly dead rhesus monkeys were recorded in our department from 1998 to 2008 years.Autopsy was performed and the pathological changes in the livers were examined and statistically analyzed according to different ages at 2 to 20 years.Results Of the 155 monkeys,88 animals had liver lesions（56.7%）.The main lesions included hepatocyte degeneration,hepatocyte necrosis,inflammatory cell infiltration,macrophage hyperplasia,liver congestion,collagen fiber hyperplasia,liver abscess,parasites,etc.Among those lesions the incidence rate of hepatocyte degeneration was the highest（34.19%）.The chi-square test shows that in the adult groups,the incidence rate of hydropic degeneration was significantly higher than that in the juvenile group,and the incidence rates of fatty degeneration,mild inflammatory cell infiltration and fibroblastic proliferation in the old age group were significantly higher than that in the juvenile and adult groups（P0.05）.Conclusions The incidence rates of spontaneous liver diseases in breeding rhesus macaques are rather high.The frequencies of hepatic pathological changes are correlated with the age of the monkeys.Attention should be paid to the spontaneous hepatic lesions when we study the liver pathology in rhesus monkeys,and old rhesus monkeys should not be used in drug safety evaluation.The results of our study provide important information of the captive monkeys,useful in quality control of captive monkeys and animal tests.