近交系小鼠过氧化氢酶-2遗传生化标记位点的研究

The Studies on Catalase-2 of Biochemical Markers of Inbred Mice

目的为了进一步完善近交系小鼠遗传生化标记检测方法,对近交系小鼠过氧化氢酶-2生化标记位点进行研究。方法将CBA/Ca与BALB/c交配得到杂交F1代动物,同时将CBA/Ca与C57BL/6交配得到杂交F1代动物,然后通过F1代动物之间的交配,以及F1代动物与母代的回交,得到F2代动物,对F2代动物进行过氧化氢酶-2生化标记检测。结果在杂交F1代不表现的过氧化氢酶-2的b型基因,在F2代出现。结论近交系小鼠过氧化氢酶-2遗传生化标记位点的等位基因a是完全显性的。

Objective To consummate genetic biochemical marker of inbred mice,we study Catalase-2 of biochemical markers of inbred mice.Methods Filial generation 1 CCBF1 were abtained by intercrossing of CBA/Ca and BALB/c;and filial generation 1 B6CBF1 were also abtained by intercrossing of CBA/Ca and C57BL/6.Filial generation 2 were obtained by intercrossing between filial generation 1 and by backcrossing between filial generation 1 and their parents.Then filial generation 2 were monitored by biochemical markers.Result Ce-2^b is detected among filial generation 2 mice,while not among filial generation 1 mice.Conclusion Ce-2^a is fully dominant.