IL-19对哮喘模型大鼠气道平滑肌细胞增殖的影响

Effect of IL-19 on Airway Smooth Muscle Cells Proliferation in Asthmatic Rats

目的探讨IL-19对哮喘大鼠气道平滑肌细胞(ASMCs)增殖的作用。方法通过对大鼠进行雾化卵清蛋白,制备哮喘模型大鼠。提取哮喘大鼠ASMCs进行培养,分别以1μg/L、10μg/L、100μg/L IL-19干预ASMCs生长。采用流式细胞仪、MTT法检测ASMCs增殖情况,观察不同浓度IL-19对ASMCs增殖的影响。结果与正常鼠相比,慢性哮喘大鼠ASMCs增殖明显,处于S期的细胞比例明显增高。经10μg/L、100μg/L IL-19干预后,慢性哮喘大鼠ASMCs处于S期的细胞比例减少,增殖亦减弱。且两组间比较有明显差异。而经1μg/L IL-19干预后,慢性哮喘大鼠ASMCs处于S期的细胞比例及增殖均无明显变化。结论一定浓度的IL-19可能抑制慢性哮喘大鼠ASMCs的增殖。

Objective To investigate the effect of IL-19 on airway smooth muscle cells（ASMCs） proliferation in asthmatic rats.Methods The rat model of asthma was made through ovalbumin（OVA） sensitization and excitation.Primary cultures of ASMCs were established for experiments.ASMCs were treated with 1 μg/L、10 μg/L 、100 μg/L IL-19.Adopt flow cytometry and MTT method to detect the proliferation of ASMCs,and observe the influence of different concentrations of IL-19 on the proliferation of ASMCs.Results Compared with normal rats,the proliferation of ASMCs in chronic asthmatic rats and the percentage of cells at S phase was obvious increased.The percentage of cells at S phase of ASMCs in chronic asthmatic rats intervened by 10 μg/L、100 μg/L IL-19 was decreased and the proliferation of ASMCs was reduced compared with the asthmatic group,and there is difference between 10 μg/L IL-19 group and 100 μg/L IL-19 group.But these two indexes of ASMCs in chronic asthmatic rats have no obvious change after intervening by 1 μg/L IL-19.Conclusion L-19 may inhibit the proliferation of ASMCs in chronic asthmatic rats.