TACE对肝癌细胞P-GP表达的影响

Effect of Transcatheter Arterial Chemoembolization on P-glycoprotein Expression in Hepatocellular Carcinoma

目的分析肝动脉栓塞化疗(TACE)对肝癌细胞多药耐药基因表达产物P糖蛋白(P-GP)的表达影响,P-GP和肝癌预后的关系,从耐药基因角度评价TACE对肝癌治疗的价值。方法采用免疫组织化学S-P法检测30例肝动脉栓塞化疗后二期切除的原发性肝癌(HCC)及癌旁组织中P-GP的表达情况,并以本课题前期研究的30例未经TACE治疗的HCC及癌旁组织中P-GP表达情况作对照。结果 TACE后P-GP在HCC和癌旁组织中阳性率分别为93.3%(28/30)、56.7%(17/30),与未做TACE的阳性率[83.3%(25/30)、30.0%(9/30)]相比,差异均有统计学意义(P<0.05)。P-GP阳性病例比P-GP阴性病例生存时间短,P-GP表达不同的肝细胞肝癌病人的生存时间有差别(P<0.05)。结论 P-GP参与介导HCC的多药耐药,TACE后肝癌细胞P-GP表达阳性率明显增高,TACE后导致继发性多药耐率增高。P-GP的表达有一定的预后判断意义。

Objective To investigate the effect of transcatheter arterial chemoembolization（TACE）on p-glycoprotein（P-GP） expression in hepatocellular carcinoma（HCC）,and to explore the relationship between P-GP and HCC prognosis and the value of TACE in the treatment of HCC.Methods The expression of P-GP in 30 cases of secondarily resected HCC and adjacent tissues after TACE（TACE group） was measured by immunohistochemistry（S-P） method and was compared with 30 cases of resected HCC and adjacent tissues managed without TACE（control group）.Results Compared with the control group,TACE significantly increased the positive rates of P-GP expression in HCC and adjacent tissues（93.3% vs 83.3% and 56.7% vs 30.0%,respectively;P〈0.05）.The average survival rate in P-GP positive patients was lower than that in P-GP negative patients（P〈0.05）.Conclusion P-GP may play an important role in multidrug resistance of HCC.TACE can increase P-GP expression and secondary resistance,and the detection of P-GP expression has value to estimate the prognosis in patients with HCC.