摘要
将人参果不同大小茎尖接种于ＭＳ＋ ６ － ＢＡ ０ ．７ｍｇ／Ｌ＋ ＩＡＡ ０ ．０２ｍｇ／Ｌ 的培养基中，３５ｄ 左右芽点长成丛生芽；丛生芽长高后，进行微型扦插，继代和长根培养基均为１／２ ＭＳ；长根的再生植株可直接移入苗床。实验还表明，接种的茎尖大小对去病毒的效果有明显影响，病毒含量不受继代培养代数的影响。
Different sizes of Solanum muricatum Ait.meristem tips were cultured in vitro on MS medium with 6-BA 0.7mg/L+IAA 0.02mg/L. After about 35 days, adventitious buds appeared from one tip. When the buds grown up, they were cut to fragments for micropropagation. The regenerating and rooting mediums were 1/2MS. The regenerated plantlets can be transferred to insectproof nursery directly. The result also showed that the size of meristem tip had obvious influence on viruses eliminating effect. The virus concentration was not changed after some generations.
关键词
人参果
茎尖培养
Solanum muricatum Ait.
Meristem tip
Virus
