黄芩苷下调沙眼衣原体感染小鼠TLR2和TLR4基因表达的研究

Baicalin downregulates expression of TLR2 and TLR4 gene in chlamydia trachomatis-infected mice

目的：研究黄芩苷对沙眼衣原体（Chlamydia trachomatis,Ct）感染小鼠宫颈组织Toll样受体2和Toll样受体4（TLR2,TLR4）基因表达的影响。方法：6~8周龄雌性C57BL/6小鼠,分为5组：阿奇霉素组,模型组,黄芩苷高、中、低剂量组。通过阴道接种Ct感染小鼠,建立Ct感染小鼠生殖道的动物模型。接种前7 d皮下注射黄体酮以增加小鼠对Ct感染的敏感性。通过比较各组小鼠阴道分泌物中的Ct数量,评价黄芩苷抗沙眼衣原体感染的能力。感染27 d之后,用RT-PCR法和Western Blot法检测宫颈组织TLR2和TLR4基因表达情况。结果：在阴道接种Ct后第3天和第6天,模型组小鼠阴道分泌物中Ct数量显著升高。通过黄芩苷治疗后,各药物组小鼠阴道分泌物中,Ct数量均明显少于模型组,黄芩苷高剂量组和阿奇霉素组显著性降低。同时,黄芩苷还可明显抑制各组宫颈组织内TLR2和TLR4基因的表达。结论：黄芩苷可有效地抑制Ct感染的小鼠宫颈组织TLR2和TLR4的高表达,这可能是黄芩苷抗Ct感染的作用机制之一。

AIM：To investigate the effects of baicalin on TLR2 and TLR4 expression in cervical tissue of gential tract Chlamydia trachomatis（Ct） infected mice. METHODS： Female C57BL/6 mice aged 6-8 weeks were divided into azithromycin group,model group,high concentrations of baicalin group,the concentration of baicalin group and low concentrations of baicalin group.Seven days before inoculation with Ct through vagina,progesterone was administered to all the experimental mice by subcutaneous injection.The numbers of Ct shedding from genital tract were evaluated between the five groups.Twenty seven days after infection,cervical tissue were taken and expression of TLR2 and TLR4 were determined by RT-PCR or Western blot.RESULTS：On the 3rd and 6th day after infection,the amount of Ct in vaginal tract in model group was higher than those in the other groups.After baicalin treatment,the amount of Ct in vaginal tract in the drug groups was lower than that in the model group.The amount of Ct in vaginal tract in the azithromycin group and high concentrations of baicalin group were significantly decreased.Meanwhile,baicalin also reduced the expression of TLR2 and TLR4 in cervical tissue.CONCLUSION： Baicalin effectively blocks high expression of TLR2 and TLR4 in cervical tissue of gential tract chlamydia-infected mice.This is probably the mechanism of baicalin in ameliorating Chlamydia trachomatis-infected mice.