丹红注射液对家兔颈动脉PTA术后内膜增生及bFGF、TF表达的影响

Effect of Danhong injection on intimal hyperplasia and expressions of bFGF and TF in carotid artery of rabbits after PTA

目的探讨丹红注射液对家兔颈动脉经皮腔内血管成形（PTA）术后内膜增生和碱性成纤维细胞生长因子（bFGF）及组织因子（TF）表达的影响。方法50只新西兰大白兔按随机数字表法分为5组：假手术组、模型组和低、中、高剂量丹红注射液治疗组，每组10只：每组再按检测时间点分为术后7d、28d2个亚组，每亚组各5只。假手术组仅结扎颈外动脉，后四组采用球囊损伤颈动脉法制备家兔颈动脉PTA术后狭窄模型。各治疗组于球囊损伤术前第1天开始分别静脉注射丹红注射液1mL／（kg·d）、2mL／（kg·d）、4mL／（kg·d），连续7d或14d。假手术组及模型组给予静脉注射生理盐水2mL／（kg·d）。术后7、28d时分别取模型靶血管进行组织学观察，免疫组化染色检测bFGF和TF的表达，图像分析系统进行半定量分析。结果术后7d时模型组新生内膜面积、新生内膜面积／中层面积（I／M）与其他组比较差异无统计学意义（P〉0．05）；术后28d时模型组新生内膜面积、I／M比值较假手术组明显增加，而中、高剂量丹红注射液治疗组较模型组均明显降低．差异有统计学意SL（P〈0．05）。与模型组相比，中、高剂量丹红注射液治疗组血管壁bFGF和TF表达量均明显降低，差异有统计学意义（P〈0．05），而低剂量丹红注射液治疗组差异无统计学意义俨〉0．05）。结论中、高剂量组丹红注射液可抑制家兔颈动脉PTA术后内膜增生，其机制可能是通过下调动脉壁bFGF和TF的表达来实现的。

Objective To study the effect of Danhong injection （DI） on intimal hyperplasia and expressions of basic fibroblast growth factor （bFGF） and tissue factor （TF） in carotid arteries of rabbits after percutaneous transluminal angioplasty（PTA）. Methods Fifty New Zealand White rabbits were randomly divided into 5 groups （n=10）, namely sham-operated group, model group, and DI treatment groups （low-, medium- and high-dose DI treatment groups）. The animals in the sham-operated group were only given external carotid artery ligation. The rabbit models of carotid stenosis in the later 4 groups were established by ballon-injury. Before the carotid balloon injury, the animals in the DI treatment groups were given DI at doses of 1 mL/kg, d-1, 2 mL/kg, d-1 and 4 mL/kg- d-1, respectively, for 7 or 14 d via intravenous injection; the animals in the sham-operated and model groups were given saline solution （2 mL/kg, d-1） instead. The animals were sacrificed and their common carotid arteries were removed on the 74 and 284 d of surgery; morphology changes of the arterial wall with hematoxylin eosin staining were observed by optical microscope, and the expressions ofbFGF and TF of the vascular wall were detected through immunohistoehemical techniques; with the help of an image analysis system, semiquantitative analysis was performed. Results On the 74 d of surgery, the intimal area and intima area/media area （I/M） ratio in the model group showed no significant differences as compared with those in the other 4 groups （P〉0.05）. On the 284 d of surgery, the intimal area and I/M ratio in the model group were increased markedly as compared with those in the sham-operated group （P〈0.05）, and the intimal area and I/M ratio in the medium and high dose DI treatment groups were significantly reduced as compared with those in the in the model group （P〈0.05）. The expressions of TF and bFGF in the neointima of carotid arteries of rabbits treated with medium and high dose of DI were significantly weaker than those of the model group （P〈0.05）, and those of rabbits treated with low dose of Dl had no obvious differences as compared with those of the model group （P〉0.05）. Conclusion Medium and high dose of DI can inhibit the intimal hyperplasia resulting from percutaneous transluminal angioplasty, might through the inhibition of expressions ofbFGF and TF in the neointima of the arterial wall.