中国西南地区丙型肝炎病毒6a亚型病毒株的传播速率

The velocity of HCV subtype 6a transmission in southwest China

目的探讨HCV6a病毒株进入中国后在中国西南部地区的播散速率。方法收集西南医院感染病专科医院2004年1月2009年9月存留的HCV6型感染患者血清，采用逆转录巢式PCR方法获取患者血清HCV CEl区序列，绘制系统进化树进行HCV亚型鉴定；用BEAST v1．6．1软件绘制时间系统进化树。结果本研究获取的HCV 6a病毒株共祖起源于1968年左右，至少存在4个主要的流行株，在静脉吸毒和非静脉吸毒患者人群中均已分离到起源较早的流行株。理论倍增曲线显示，1997年以后HCV 6a病毒传播速率加快，2007年以后加速明显。结论HCV 6a病毒株在中国西南部地区播散迅速，在未来有可能成为本地区主要的HCV亚型，并有由静脉吸毒人群向非静脉吸毒人群传播的趋势。

Objective To estimate the velocity of HCV subtype 6a transmission in Southwest China. Methods The HCV CE 1 region from 61 patients infected with HCV genotype 6 were amplificated by RT- PCR and sequenced. The subtypes were identified, and the period of HCV 6a strains originated in southwest china was estimated by using molecular clock phylogenetic analysis. The velocity of HCV subtype 6a transmission in southwest China was estimated by BEAST vl.6.1 and Tracer vl.5 software theoretically. Reslut Most of HCV 6a strains distributed in Southwest China orgined around the year 1968 and at last 4 epidemic strains existed. The earlier orgined strains could be isolated both in intravenous drug users （IDU） and non- IDU patients. After 1997, the HCV 6a strains transmission in southwest China accelerated and the trend intensified in 2007. Conclusion HCV 6a strains spread lastly both in IDU and non-IDU patients, which might be the main HCV subtype distributed in Southwest China in the future.