22种木莲属植物亲缘关系的ISSR分析

Analyses of the Genetic Relationships Among 22 Species of Manglietia Plants Using ISSR Markers

利用ISSR标记分析技术探讨了22种木莲属植物的遗传亲缘关系。选用10对ISSR引物组合对木莲属22个种的基因组进行分析。结果表明:22个种共扩增出595条DNA带,其中多态性条带占94.45%;根据Nei-Li遗传相似性系数在0.68处,UPGMA聚类结果将22个种划分为6个类群,在0.70处进一步可分为12个亚类群;22种木莲属植物的Nei-Li遗传相似性系数变化范围为0.605(乳源木莲与球果木莲之间)～0.956(中缅木莲与滇南木莲之间),平均遗传相似性系数为0.698。说明木莲属植物种间差异性大。其中中缅木莲、滇南木莲之间遗传相似性系数为0.956,两者亲缘关系最近。该结果同时支持将巴东木莲、乳源木莲、滇桂木莲分别作为独立的种。

The genetic relationships among 22 species of Manglietia plants were analyzed by using inter-simple sequence repeat（ISSR） molecular-marked technique.Ten ISSR primers were selected to assess the genomes of 22 species of Manglietia plants.The result showed that a total of 595 DNA bands were amplified and 562 of which（94.45%） were polymorphic.According to the Nei-Li genetic similarity of 0.68,UPGMA method cluster analysis indicated that these 22 species were classified into 6 cluster groups,and they were classified into 12 sub-cluster groups with the genetic similarity of 0.70.The Nei-Li genetic similarities of 22 species of Manglietia ranged from 0.605（between M.yuyuanensis and M.conifera） to 0.956（between M.hookeri and M.wangii）.The average genetic similarity is 0.698,which suggests that Manglietia have a plenty of inter-species diversity.The genetic similarity coefficient between M.hookeri and M.wangii is 0.956,and their genetic relationship is actually the closest.The result supports the classification of M.patungensis,M.yuyuanensis and M.forrestii as independent species respectively.