UAE-IC法测定大白口蘑中阴离子含量

Determination of anions in Tricholoma giganteum fruiting body by ultrasonic assisted extraction-ion chromatography

采用超声波(UAE)辅助提取-离子色谱(IC)法测定了大白口蘑中F-、Cl-、NO2-、NO3-、PO43-5种阴离子。选用NaOH溶液(3.0mmol/L)超声渗提30min后进样100μL,通过流速为1.2mL/min的淋洗液Na2CO3+NaHCO3(3.5mmol/L+1.0mmol/L)洗脱。F-、Cl-、NO2-、NO3-、PO43-5种离子线性范围分别为2-25mg/L、2-20mg/L、2-100mg/L、10-55mg/L、10-100mg/L,峰面积RSD分别为0.58%、1.27%、0.73%、0.92%、2.33%,检出限在0.0976-1.0984mg/L之间。大白口蘑样品中未检出NO2-,其余F-、Cl-、NO3-、PO43-4种离子加标回收率在95%-110%之间。

Five anions （F-,Cl-,NO2-,PO43-,NO3-） in Tricholoma giganteum fruiting body were determined by using ultrasonic assisted extraction （UAE）-ion chromatography （IC）.After ultrasonic assisted extraction for 30min in NaOH solvent （3.0mmol/L）,sample introduction as 100μL,Na2CO3＋NaHCO3 （3.5mmol/L＋1.0mmol/L） were chosen as washing solution with flowing rate at 1.2mL/min.The linearity ranges of 5 anions respectively were 2-25mg/L,2-20mg/L,2-100mg/L,10-55mg/L,10-100mg/L.The relative standard deviations （RSD） of the peak areas were 0.58%,1.27%,0.73%,0.92%,2.33%,respectively.The detection limits of the 5 anions were 0.0976-1.0984mg/L.The spike recoveries of 4 anions （F-,Cl-,PO43-,NO3-） were 95%-110%,whereas,the content of NO2- in T.giganteum was not detected out.