CO1在侧耳属物种快速鉴定中的应用

Application of CO1 for rapid identification of Pleurotus species

以侧耳属Pleurotus15个种的15个菌株为材料,根据GenBank上侧耳属细胞色素c氧化酶亚基Ⅰ基因(cytochrome c oxidase subunit1gene,CO1)序列信息,设计引物CO332F、CO332R,进行第一轮PCR扩增,结果显示所有菌株都能得到单一条带,根据条带大小,15个菌株可分为4组。随后针对每个种设计特异性引物,进行第二轮PCR扩增,结果显示每个菌株只有在自己特异的引物中出现目的条带。通过两轮扩增,根据扩增条带的大小和有无,即可对15个种进行快速鉴定。

15 strains belonging to 15 species of Pleurotus were used.Primer CO332F and CO332R were designed by the cytochrome c oxidase subunit 1 gene （CO1） sequence of Pleurotus downloaded from GenBank.Results of the first-step PCR amplification showed that all the strains can get one band.The 15 strains were divided into four groups by the band size.Species-specific primers were designed accordingly.Results of the second-step PCR amplification showed that each species can get one band by the species-specific primers.In conclusion,the 15 species of Pleurotus can be identified by two-step PCR in accordance with the band size and the presence or absence of the band.